Effects of the steric hindrance of micropores in the hyper-cross-linked polymeric adsorbent on the adsorption of p-nitroaniline in aqueous solution
作者：
Xiao, Guqing* ;Wen, Ruimin;Wei, Dongmei;Wu, Dan
期刊：
Journal of Hazardous Materials ,2014年280:97-103 ISSN：0304-3894
通讯作者：
Xiao, Guqing
作者机构：
[Wen, Ruimin; Xiao, Guqing; Wei, Dongmei; Wu, Dan] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Xiao, Guqing] H;Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
关键词：
Adsorption;Hyper-cross-linked polymeric adsorbent;Micropore;Steric hindrance;p-Nitroaniline
摘要：
A hyper-cross-linked polymeric adsorbent with "CH<inf>2</inf>phenolCH<inf>2</inf>" as the cross-linked bridge (denoted GQ-05), and another hyper-cross-linked polymeric adsorbent with "CH<inf>2</inf>p-cresolCH<inf>2</inf>" as the cross-linked bridge (denoted GQ-03) were synthesized to reveal the effect of the steric hindrance of micropores in the hyper-cross-linked polymeric adsorbent on adsorption capacity and adsorption rate of p-nitroaniline (PNA) from aqueous solution. The results of adsorption kinetics indicated the order of the adsorption rate GQ-05>GQ-03. The pseudo-first-order rate equation could describe the entire adsorption process of PNA onto GQ-05 while the equation characterized the adsorption process of GQ-03 in two stages. The order of the adsorption capacity GQ-05>GQ-03 was demonstrated by thermodynamic analysis and dynamic adsorption. The steric hindrance of micropores in the hyper-cross-linked polymeric adsorbent was a crucial factor for the order of the adsorption capacity and adsorption rate. ©2014 Elsevier B.V.
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英文
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Water extract of the fungi from Fuzhuan brick tea improves the beneficial function on inhibiting fat deposition
作者：
Peng, Yuxuan;Xiong, Zhe;Li, Juan;Huang, Jian-an;Teng, Cuiqin;...
期刊：
International Journal of Food Sciences and Nutrition ,2014年65(5):610-614 ISSN：0963-7486
通讯作者：
Gong, Yushun
作者机构：
[Peng, Yuxuan; Huang, Jian-an; Gong, Yushun; Li, Juan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuxuan] Hunan City Univ, Sch Chem & Environm Engn, Yiyang, Peoples R China.;[Xiong, Zhe; Teng, Cuiqin; Liu, Zhonghua] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Gong, Yushun] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
3T3-L1 preadipocyte;Caenorhabditis elegans;Eurotium cristatum;Fuzhuan brick tea;fat deposition
摘要：
Fuzhuan brick tea (FBT) is traditionally consumed by the ethnic group in the border region of northwest China. The unique yellow fungal (Eurotium cristatum) growth phase is considered to be the key process point in the manufacture of the brick tea. The fungi from FBT are not only strongly correlated to the quality of brick tea, but also have the potential function of preventing obesity. The water extract of fungi (100 μg/mL) can significantly inhibit fat deposition in 3T3-L1 adipocyte and Caenorhabditis elegans. Furthermore, the inhibition of 3T3-L1 adipocyte formation was not due to the suppression on cell viability. © 2014 Informa UK Ltd. All rights reserved.
语种：
英文
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Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting
作者：
Chen, Yaoning* ;Zhou, Wei;Li, Yuanping;Zhang, Jiachao;Zeng, Guangming;...
期刊：
Applied Microbiology and Biotechnology ,2014年98(9):4233-4243 ISSN：0175-7598
通讯作者：
Chen, Yaoning
作者机构：
[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.;[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China.;[Li, Yuanping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.;[Zhang, Jiachao] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen, Yaoning] H;Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.
关键词：
Composting;PCR-DGGE;Denitrifying bacteria;RDA;Phylogenetic analysis
摘要：
The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high diversity of nir gene sequences within composting samples. RDA showed that the nirK and nirS gene structures were significantly related to pH and pile temperature (P < 0.05). Significant amounts of the variation (49.2 and 38.3 % for nirK and nirS genes, respectively) were explained by pH and pile temperature, suggesting that those two parameters were the most likely ones to influence, or be influenced by the denitrifiers harboring nirK and nirS genes. © 2014 Springer-Verlag.
语种：
英文
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Synthesis, spectroscopic properties and theoretical studies of bis-Schiff bases derived from polyamine and pyrazolones
作者：
Ren, Tiegang* ;Liu, Shuyun;Li, Guihui;Zhang, Jinglai;Guo, Jia;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2012年97:167-175 ISSN：1386-1425
通讯作者：
Ren, Tiegang
作者机构：
[Yang, Lirong; Ren, Tiegang; Zhang, Jinglai; Li, Weijie; Guo, Jia] Henan Univ, Coll Chem & Chem Engn, Fine Chem & Engn Res Inst, Kaifeng 475004, Peoples R China.;[Liu, Shuyun] Hunan City Univ, Yiyang 413000, Peoples R China.;[Li, Guihui] Henan Univ, Key Lab, Minist Educ Special Funct Mat, Kaifeng 475004, Peoples R China.
通讯机构：
[Ren, Tiegang] H;Henan Univ, Coll Chem & Chem Engn, Fine Chem & Engn Res Inst, Kaifeng 475004, Peoples R China.
关键词：
Polyamine;Pyrazolone;Schiff base;Spectroscopic properties;Synthesis;Theoretical studies
摘要：
A series of novel bis-Schiff base were synthesized from 1-aryl-3-methyl-4-benzoyl-5-pyrazolones and diethylenetriamine (or triethylenetetramine) as the starting materials. All of these bis-Schiff bases were characterized by means of NMR, IR, and MS. The UV-vis absorption spectra and fluorescent spectra of these bis-Schiff bases were also measured. Moreover, the B3LYP/6-31G(d) method was used to optimize the ground state geometry of the bis-Schiff bases; and the UV-vis spectroscopic properties of the products were computed and compared with corresponding experimental data based on cc-pVDZ basis set of TD-B3LYP method. It has been found that all of these bis-Schiff bases show a remarkable absorption peak in a wavelength range of 270-340 nm; and their maximum emission peaks are around 348 nm.
语种：
英文
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Construction of gene library of 20 kb DNAs from parasporal crystal in bacillus thuringiensis strain 4.0718: Phylogenetic analysis and molecular docking
作者：
Wu, Feng;Zhao, Xinmin;Sun, Yunjun;Li, Wenping;Xia, Liqiu* ;...
期刊：
Current Microbiology ,2012年64(2):106-111 ISSN：0343-8651
通讯作者：
Xia, Liqiu
作者机构：
[Wu, Feng; Li, Wenping; Yin, Jia; Zhao, Xinmin; Hu, Shengbiao; Yu, Ziquan; Sun, Yunjun; Xia, Liqiu; Ding, Xuezhi; Tang, Ying] Hunan Normal Univ, Hunan Prov Key Lab Microbial Mol Biol, State Key Lab Breeding Base Microbial Mol Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Zhao, Xinmin] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.
通讯机构：
[Xia, Liqiu] H;Hunan Normal Univ, Hunan Prov Key Lab Microbial Mol Biol, State Key Lab Breeding Base Microbial Mol Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
Bacillus thuringiensis;20 kb DNAs;Gene library;Homology analysis;Phylogenetic analysis;Molecular docking
摘要：
The 20 kb DNAs are associated with crystals in many subspecies of Bacillus thuringiensis. We isolated 20 kb DNAs from crystals of B. thuringiensis strain 4.0718, then constructed a gene library using DNA fragments of Sau3AI partial digestion and pbluescriptIISK(+) vector. We screened out 440 recombinants, yielding a genomic coverage of ten and including 99% sequence of DNA which achieved the required theoretical value to construct the gene library. Through NCBI Blast and homology analysis, the sequencing results proved that the DNA came from the chromosome of B. thuringiensis. Moreover, we have completed the multiple alignment of homologous ropB protein sequences and phylogenetic analysis using bioinformatic software. For further investigation of the interactions between 20 kb DNAs and protoxins, molecular docking has also been done.
语种：
英文
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A cry1ac toxin variant generated by directed evolution has enhanced toxicity against lepidopteran insects
作者：
Shan, Shiping;Zhang, Youming;Ding, Xuezhi;Hu, Shengbiao;Sun, Yunjun;...
期刊：
Current Microbiology ,2011年62(2):358-365 ISSN：0343-8651
通讯作者：
Xia, Liqiu
作者机构：
[Zhang, Youming; Hu, Shengbiao; Yu, Ziquan; Zhu, Zhou; Sun, Yunjun; Xia, Liqiu; Shan, Shiping; Ding, Xuezhi] Hunan Normal Univ, Key Lab Microbial Mol Biol Hunan Prov, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Liu, Shiquan] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.
通讯机构：
[Xia, Liqiu] H;Hunan Normal Univ, Key Lab Microbial Mol Biol Hunan Prov, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
Bacillus Thuringiensis;Insecticidal Activity;Shanghai Sangon Biological Engineer Technology;cry1Ac Gene;Insecticidal Crystal Protein
摘要：
Cry1Ac insecticidal crystal proteins produced by Bacillus thuringiensis (Bt) have become an important natural biological agent for the control of lepidopteran insects. In this study, a cry1Ac toxin gene from Bacillus thuringiensis 4.0718 was modified by using error-prone PCR, staggered extension process (StEP) shuffling combined with Red/ET homologous recombination to investigate the insecticidal activity of delta-endotoxin Cry1Ac. A Cry1Ac toxin variant (designated as T524N) screened by insect bioassay showed increased insecticidal activity against Spodoptera exigua larvae while its original insecticidal activity against Helicoverpa armigera larvae was still retained. The mutant toxin T524N had one amino acid substitution at position 524 relative to the original Cry1Ac toxin, and it can accumulate within the acrystalliferous strain Cry-B and form more but a little smaller bipyramidal crystals than the original Cry1Ac toxin. Analysis of theoretical molecular models of mutant and original Cry1Ac proteins indicated that the mutation T524N located in the loop linking β16-β17 of domain III in Cry1Ac toxin happens in the fourth conserved block which is an arginine-rich region to form a highly hydrophobic surface involving interaction with receptor molecules. This study showed for the first time that single mutation T524N played an essential role in the insecticidal activity. This finding provides the biological evidence of the structural function of domain III in insecticidal activity of the Cry1Ac toxin, which probably leads to a deep understanding between the interaction of toxic proteins and receptor macromolecules. © 2010 Springer Science+Business Media, LLC.
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英文
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The Theoretical Three-dimensional Structure of Bacillus thuringiensis Cry5Aa and Its Biological Implications
作者：
Xin-Min, Zhao;Li-Qiu, Xia* ;Xue-Zhi, Ding;Fa-Xiang, Wang
期刊：
PROTEIN JOURNAL ,2009年28(2):104-110 ISSN：1572-3887
通讯作者：
Li-Qiu, Xia
作者机构：
[Li-Qiu, Xia; Xue-Zhi, Ding; Xin-Min, Zhao; Fa-Xiang, Wang] Hunan Normal Univ, Key Lab Microbial Mol Biol Hunan Prov, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Xin-Min, Zhao] Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Li-Qiu, Xia] H;Hunan Normal Univ, Key Lab Microbial Mol Biol Hunan Prov, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
Three-dimensional structure;Homology modeling;Cry5Aa;Bacillus thuringiensis
摘要：
Cry5Aa is a crystal protein produced by Bacillus thuringiensis serovar. damstadiensis during its stationary phase, this δ-endotoxin is active against nematodes and has great potential for nematodes control. The theoretical model of the three-dimensional structure of Cry5Aa was predicted by homology modeling on the structures of the Cry1Aa which is specific to Lepidopteran insects. The structure of the Cry5Aa resembles previously reported Cry toxin structures but shows the following distinctions. Cry5Aa has a long insertion in α2 of domain I. Some loops in the domain II and III of Cry5Aa are exposed to the solvent. In this work we give a brief description of our model and hypothesize the residues of the Cry5Aa that could be important in receptor recognition and pore formation. This model will be helpful for the design of mutagenesis experiments aimed to the improvement of toxicity, and lead to a deep understanding of the mechanism of action of nematicidal toxins. © 2009 Springer Science+Business Media, LLC.
语种：
英文
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Reversible molecular switching of molecular beacon: controlling DNA hybridization kinetics and thermodynamics using mercury(II) ions
作者：
Yang, Ronghua* ;Jin, Jianyu;Long, Liping;Wang, Yongxiang;Wang, Hao;...
期刊：
Chemical Communications ,2009年(3):322-324 ISSN：1359-7345
通讯作者：
Yang, Ronghua
作者机构：
[Yang, Ronghua; Long, Liping; Tan, Weihong] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Ronghua; Long, Liping; Tan, Weihong] Hunan Univ, Ctr Biomed Engn, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.;[Jin, Jianyu; Wang, Yongxiang; Yang, Ronghua; Wang, Hao] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China.;[Long, Liping] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413500, Hunan, Peoples R China.;[Tan, Weihong] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA.
通讯机构：
[Yang, Ronghua] H;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
We report that the hydrogen-bonding pattern in a molecular beacon can be replaced by metal-dependent pairs of Hg 2+ and DNA thymine (T) bases. A molecular beacon based on T-Hg 2+ -T exhibits a lower background signal and higher thermostability than regular molecular beacons. © The Royal Society of Chemistry.
语种：
英文
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The theoretical 3D structure of Bacillus thuringiensis Cry5Ba
作者：
Xia, Li-Qiu* ;Zhao, Xin-Min;Ding, Xue-Zhi;Wang, Fa-Xiang;Sun, Yun-Jun
期刊：
Journal of Molecular Modeling ,2008年14(9):843-848 ISSN：1610-2940
通讯作者：
Xia, Li-Qiu
作者机构：
[Ding, Xue-Zhi; Xia, Li-Qiu; Sun, Yun-Jun; Wang, Fa-Xiang] Hunan Normal Univ, Coll Life Sci, Key Lab Microbial Mol Biol Hunan Prov, Changsha 410081, Peoples R China.;[Zhao, Xin-Min] Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Xia, Li-Qiu] H;Hunan Normal Univ, Coll Life Sci, Key Lab Microbial Mol Biol Hunan Prov, Changsha 410081, Peoples R China.
关键词：
Three-dimensional structure;Homology modeling;Cry5Ba;Bacillus thuringiensis
摘要：
Cry5Ba is a delta-endotoxin produced by Bacillus thuringiensis PS86A1 NRRL B-18900. It is active against nematodes and has great potential for nematode control. Here, we predict the first theoretical model of the three-dimensional (3D) structure of a Cry5Ba toxin by homology modeling on the structure of the Cry1Aa toxin, which is specific to Lepidopteran insects. Cry5Ba resembles the previously reported Cry1Aa toxin structure in that they share a common 3D structure with three domains, but there are some distinctions, with the main differences being located in the loops of domain I. Cry5Ba exhibits a changeable extending conformation structure, and this special structure may also be involved in pore-forming and specificity determination. A fuller understanding of the 3D structure will be helpful in the design of mutagenesis experiments aimed at improving toxicity, and lead to a deep understanding of the mechanism of action of nematicidal toxins.
语种：
英文
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Interactions between protein and porphyrin-containing cyclodextrin supramolecular system: a fluorescent sensing approach for albumin
作者：
Long, Liping;Jin, Man Yu;Zhang, Ying;Yang, Ronghua* ;Wang, Keming
期刊：
ANALYST ,2008年133(9):1201-1208 ISSN：0003-2654
通讯作者：
Yang, Ronghua
作者机构：
[Yang, Ronghua; Jin, Man Yu; Zhang, Ying; Long, Liping] Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China.;[Wang, Keming; Yang, Ronghua] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Long, Liping] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413500, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] P;Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China.
关键词：
BETA-CYCLODEXTRIN;COMPLEXES;TETRAPHENYLPORPHYRIN;MEMBRANE;RECOGNITION;SENSORS;BINDING;AMINES;STATE;DIMER
摘要：
The interactions of meso-tetraphenylporphyrin (TPP), meso-tetraphenylporphyrin cobalt(II) (CoTPP) and protein in the presence of a cyclodextrin derivative, heptakis(2,6-di-O-n-octyl)-beta-cyclodextrin (Oc-beta-CD), have been investigated. In the presence of Oc-beta-CD, significant increase of TPP fluorescence was realized, but the increased fluorescence was quenched by CoTPP. To further investigate the fluorescence-quenched system and explore its potential application in bioanalysis, a strategy has been devised to restore the quenching fluorescence of TPP upon interacting with protein. The restoration of TPP fluorescence in the present system is fast and accomplished upon interaction with bovine serum albumin (BSA) or human serum albumin (HSA). On the basis of the spectroscopic measurement and excited state fluorescence lifetime, the mechanism of TPP fluorescence quenching is attributed to formation of a ground-state complex of TPP and CoTPP, and the fluorescence restoration is attributed to the binding of CoTPP with the protein molecule which destroys the aggregate, releasing the free base porphyrin. With optimized conditions, the calibration equations are linear from 0.80 to 75.4 mu g mL(-1) BSA and from 3.20 to 93.2 mu g mL(-1) HSA. The corresponding detection limits are 0.32 mu g mL(-1) for BSA and 1.06 mu g mL(-1) for HSA, respectively The method was used for the direct assay of HSA content in human serum. The result is comparable to that obtained by another method. The recovery from BSA in synthetic sample is also satisfactory.
语种：
英文
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霍山石斛不同生长阶段遗传稳定性的RAPD分析
作者：
刘石泉;李小军;余庆波;谢红;周根余
期刊：
中国中药杂志 ,2007年32(10):902-905 ISSN：1001-5302
通讯作者：
Liu, S.-Q.
作者机构：
[刘石泉] 湖南城市学院化学与环境工程系;[李小军; 余庆波; 谢红; 周根余] 上海师范大学生命与环境科学学院生物系遗传学实验室
通讯机构：
Department of Chemistry and Environment Engineering, Hunan City University, China
关键词：
随机扩增多态性DNA;霍山石斛;生长阶段;遗传稳定性
摘要：
目的：研究霍山石斛同一蒴果组培不同生长阶段遗传稳定性。方法：利用从20个随机引物筛选的3个稳定性较好的10碱基引物对已继代7-8次的H23种群进行RAPD检测。结果：发现不同阶段内遗传相似系数较大,在85.409 3%-98.360 6%,其中在原球茎、萌芽期和一叶期存在的变异比两叶期、开花期要显著,但程度都极其微弱。结论：来自同一蒴果的变异非常小,建立霍山石斛稳定的无性快繁系是切实可行的。
语种：
中文
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Silica Sol-gel Amperometric Immunosensor for Schistosoma Japonicum Antibody Assay
作者：
Zhong, TS;Liu, GD*
期刊：
Analytical Sciences ,2004年20(3):537-541 ISSN：0910-6340
通讯作者：
Liu, GD
作者机构：
[Liu, GD] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA.;Hunan City Coll, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China.
通讯机构：
[Liu, GD] N;New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA.
摘要：
An amperometric immunosensor was constructed by dispersing graphite, schistosoma-japonicum antigen (SjAg) and silica sol-gel at low temperature. The performance characteristics of the prepared immunosensor were examined in the buffered solution of o-aminophenol (o-AP) used as a substrate. It exhibited excellent physical and electrochemical stability with a renewable external surface. A competitive binding assay was employed to determine schistosoma-japonicum antibody (SjAb) with the aid of horseradish peroxidase labeled SjAb (HRP-SjAb). The experimental parameters for SjAb assay were optimized, including the amount of labeled SjAb in incubation solution, incubation time, temperature and the pH of solution. The use of o-AP substrate and amperometric detection at -250 mV (vs. SCE) results in a determination limit of 0.32 μg/ml and a linear range extending up to 0.18 μg/ml. The results of SjAb assay in serum samples demonstrate the feasibility of using the proposed immunosensor for clinical analysis.
语种：
英文
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An amperometric hydrogen peroxide biosensor based on immobilizing horseradish peroxidase to a nano-Au monolayer supported by sol–gel derived carbon ceramic electrode
作者：
Lei, CX;Hu, SQ;Gao, N;Shen, GL;Yu, RQ*
期刊：
Bioelectrochemistry ,2004年65(1):33-39 ISSN：1567-5394
通讯作者：
Yu, RQ
作者机构：
[Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.;Zhuzhou Teachers Coll, Dept Chem, Zhuzhou 412007, Peoples R China.
通讯机构：
[Yu, RQ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
Amperometric biosensor;Horseradish peroxidase;Hydrogen peroxide;Nano-Au monolayer
摘要：
A novel strategy for fabricating horseradish peroxidase (HRP)-based H 2O 2 sensor has been developed by combining the merits of carbon sol-gel supporting matrix and nano-scaled particulate gold (nano-Au) mediator. The thiol functional group-derived carbon ceramic electrode (CCE) was first constructed using (3-mercaptopropyl) trimethoxy silane as sol-gel monomer. Then, the stable nano-Au monolayer was obtained through covalent linkage between nano-Au and thiol group on the surface of CCE. The experimental results showed that nano-Au monolayer formed not only could steadily immobilize HRP but also efficiently retain its bioactivity. Hydrogen peroxide was detected with the aid of hydroquinone mediator to transfer electrons between the electrode and HRP. The process parameters for the fabrication of the enzyme electrode and various experimental variables such as the operating potential, mediator concentration and pH of background electrolyte were explored for optimum analytical performance of the enzyme electrode. The biosensor had a fast response of less than 8 s with linear range of 1.22×10 -5 to 1.10×10 -3mol l -1 and a detection limit of 6.1×10 -6mol l -1. The sensitivity of the sensor for H 2O 2 was 0.29 A l mol -1 cm -2. The activation energy for enzyme reaction was calculated to be 10.1 kJ mol -1. The enzyme electrode retained 75% of its initial activity after 5 weeks storage in phosphate buffer at pH 7. © 2004 Elsevier B.V. All rights reserved.
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英文
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