An amperometric hydrogen peroxide biosensor based on immobilizing horseradish peroxidase to a nano-Au monolayer supported by sol–gel derived carbon ceramic electrode
This work was supported by the National Natural Science Foundation of China (Grants No. 29975006, 20075006), the Foundation for PhD Thesis Research (No. 20010532008), and the Foundation of Science Commission of Hunan Province.
机构署名：
本校为其他机构
院系归属：
材料与化学工程学院
摘要：
A novel strategy for fabricating horseradish peroxidase (HRP)-based H 2O2 sensor has been developed by combining the merits of carbon sol-gel supporting matrix and nano-scaled particulate gold (nano-Au) mediator. The thiol functional group-derived carbon ceramic electrode (CCE) was first constructed using (3-mercaptopropyl) trimethoxy silane as sol-gel monomer. Then, the stable nano-Au monolayer was obtained through covalent linkage between nano-Au and thiol group on the surface of CCE. The experimental results showed that nano-Au monolayer formed not only could steadily immobilize HRP but als...
