National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2006AA02Z187, 2006AA10A212]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30670052]; Ph.D. Programs Foundation of Ministry of Education of ChinaMinistry of Education, China [20060452006]
机构署名：
本校为其他机构
院系归属：
材料与化学工程学院
摘要：
Cry1Ac insecticidal crystal proteins produced by Bacillus thuringiensis (Bt) have become an important natural biological agent for the control of lepidopteran insects. In this study, a cry1Ac toxin gene from Bacillus thuringiensis 4.0718 was modified by using error-prone PCR, staggered extension process (StEP) shuffling combined with Red/ET homologous recombination to investigate the insecticidal activity of delta-endotoxin Cry1Ac. A Cry1Ac toxin variant (designated as T524N) screened by insect bioassay showed increased insecticidal activity ag...
