Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting

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成果类型：

期刊论文

作者：

Chen, Yaoning*;Zhou, Wei;Li, Yuanping;Zhang, Jiachao;Zeng, Guangming;...

通讯作者：

Chen, Yaoning

作者机构：

[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.

[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China.

[Li, Yuanping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.

[Zhang, Jiachao] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Chen, Yaoning] H

Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.

语种：

英文

关键词：

Composting;PCR-DGGE;Denitrifying bacteria;RDA;Phylogenetic analysis

期刊：

Applied Microbiology and Biotechnology

ISSN：

0175-7598

年：

2014

卷：

期：

页码：

4233-4243

DOI：

10.1007/s00253-014-5514-0

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [50808072, 50978088, 50908078]; Hunan Key Scientific Research Project [2009FJ1010]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [13JJB002]

机构署名：

本校为其他机构

院系归属：

材料与化学工程学院

摘要：

The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high dive...

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Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting

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