期刊论文

Liu, S.-Q.

中文

中国中药杂志

1001-5302

2007

32

10

902-905

10.3321/j.issn:1001-5302.2007.10.007

湖南省高等学校科学研究项目（06C218）；

本校为第一且通讯机构

目的：研究霍山石斛同一蒴果组培不同生长阶段遗传稳定性。方法：利用从20个随机引物筛选的3个稳定性较好的10碱基引物对已继代7-8次的H23种群进行RAPD检测。结果：发现不同阶段内遗传相似系数较大,在85.409 3%-98.360 6%,其中在原球茎、萌芽期和一叶期存在的变异比两叶期、开花期要显著,但程度都极其微弱。结论：来自同一蒴果的变异非常小,建立霍山石斛稳定的无性快繁系是切实可行的。

Objective: To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system. Method: Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification , random amplified polymorphic DNA (RAPD) were performed. Result: The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence. Conclusion: The variation of the same pod is quite small. It...