作者： Lei, Cun-Xi;Hu, Shun-Qin;Shen, Guo-Li;Yu, Ru-Qin

期刊： Talanta,2003年59(5):981-988 ISSN：0039-9140

通讯作者： Yu, RQ

作者机构： [Yu, Ru-Qin] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Coll, Dept Chem, Yiyang 413049, Peoples R China.;Zhuzhou Teachers Coll, Dept Chem, Zhuzhou 412007, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Nano-Au monolayer;Chitosan-entrapped carbon paste electrode;Hydrogen peroxide;Enzyme sensor

摘要： A procedure for fabricating an enzyme electrode has been described based on the effective immobilization of horseradish peroxidase (HRP) to a nano-scaled particulate gold (nano-Au) monolayer modified chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups has been utilized to realize the use of nano-Au as an intermediator to retain high bioactivity of the enzyme. Hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) was determined in the presence of hydroquinone as a mediator to transfer electrons between the electrode and HRP. The HRP immobilized on nano-Au displayed excellent electrocatalytical activity to the reduction of H<inf>2</inf>O<inf>2</inf>. The effects of experimental variables such as the operating potential of the working electrode, mediator concentration and pH of measuring solution were investigated for optimum analytical performance by using an amperometric method. The enzyme electrode provided a linear response to hydrogen peroxide over a concentration range of 1.22 &times;10^-5 -2.43 &times;10^-3 mol 1^-1 with a sensitivity of 0.013 A 1 mol^-1 cm^-2 and a detection limit of 6.3 &mu;mol 1^-1 based on signal per noise = 3. The apparent Michaelis-Menten constant (K<inf>m</inf>^app) for the sensor was found to be 0.36 mmol 1^-1. The lifetime, fabrication reproducibility and measurement repeatability were evaluated with satisfactory results. The analysis results of real sample by this sensor were in satisfactory agreement with those of the potassium permanganate titration method. &copy;2003 Elsevier Science B.V. All rights reserved.

语种： 英文

作者： Lei, CX*;Long, LP;Can, ZL

期刊： ANALYTICAL LETTERS,2005年38(11):1721-1734 ISSN：0003-2719

通讯作者： Lei, CX

作者机构： [Lei, CX; Long, LP; Can, ZL] Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China.

通讯机构： [Lei, CX] Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China.

关键词： amperometric biosensor;silica sol-gel/alginate composite material;HRP-nano-Au;hydrogen peroxide

摘要： A novel approach to assemble an H2O2 amperometric biosensor was introduced. The biosensor was constructed by entrapping horseradish peroxidase (HRP) labeled nano-scaled particulate gold (nano-Au) (HRP-nano-Au electrostatic composite) in a new silica sol-gel/alginate hybrid film using glassy carbon electrode as based electrode. This suggested strategy fully merged the merits of sol-gel derived inorganic-organic composite film and the nano-Au intermediator. The silica sol-gel/alginate hybrid material can improve the properties of conventional sol-gel material and effectively prevent cracking of film. The entrapment of HRP in the form of HRP-nano-Au can not only factually prevent the leaking of enzyme out of the film but also provide a favorable microenvironment for HRP. With hydroquinone as an electron mediator, the proposed HRP electrode exhibited good catalytic activity for the reduction of H2O2. The parameters affecting both the qualities of sol-gel/alginate hybrid film and the biosensor response were optimized. The biosensor exhibited high sensitivity of 0.40 Al mol-1 cm-2 for H2O2 over a wide linear range of concentration from 1.22 × 10-5 to 1.46 × 10-3 mol L-1, rapid response of < 5 s and a detection limit of 0.61 × 10 -6 mol L-1. The enzyme electrode has remarkable stability and retained 86% of its initial activity after 45 days of storage in 0.1 mol L-1 Tris-HCl buffer solutions at pH 7. Copyright © Taylor & Francis, Inc.

语种： 英文

作者： Lei, Cun‐Xi;Wang, Hua;Shen, Guo‐Li;Yu, Ru‐Qin

期刊： Electroanalysis,2004年16(9):736-740 ISSN：1521-4109

通讯作者： Yu, RQ

作者机构： [Yu, Ru‐Qin] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Coll, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Enzyme sensor;Nano‐Au film;Glassy carbon electrode;Hydrogen peroxide;Glucose

摘要： A new enzyme‐based amperometric biosensor for hydrogen peroxide was developed relying on the efficient immobilization of horseradish peroxidase (HRP) to a nano‐scaled particulate gold (nano‐Au) film modified glassy carbon electrode (GC). The nano‐Au film was obtained by a chitosan film which was first formed on the surface of GC. The high affinity of chitosan for nano‐Au associated with its amino groups resulted in the formation of nano‐Au film on the surface of GC. The film formed served as an intermediator to retain high efficient and stable immobilization of the enzyme. H2O2 was detected using hydroquinone as an electron mediator to transfer electrons between the electrode and HRP. The HRP immobilized on nano‐Au film maintained excellent electrocatalytical activity to the reduction of H2O2. The experimental parameters such as the operating potential of the working electrode, mediator concentration and pH of background electrolyte were optimized for best analytical performance of amperometry. The linear range of detection for H2O2 is from 6.1×10−6 to 1.8×10−3 mol L−1 with a detection limit of 6.1 μmol L−1 based on signal/noise=3. The proposed HRP enzyme sensor has the features of high sensitivity (0.25 Almol−1cm−2), fast response time (t90%≤10 s) and a long‐term stability (>1 month). As an extension, glucose oxidase (GOD) was chemically bound to HRP‐modified electrode. A GOD/HRP bienzyme‐modified electrode formed in this way can be applied to the determination of glucose with satisfactory performance.

语种： 英文

作者： Lei, Cun-Xi;Gong, Fu-Chun;Shen, Guo-Li;Yu, Ru-Qin

期刊： Sensors and Actuators, B: Chemical,2003年96(3):582-588 ISSN：0925-4005

通讯作者： Yu, RQ

作者机构： [Yu, Ru-Qin] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Coll, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： amperometric immunosensor;nano-Au monolayer;chitosan-entrapped carbon paste electrode;Schistosoma japonicum

摘要： A new strategy to construct amperometric immunosensor for Schistosoma japonicum antigen (SjAg) assay based on nano-size particulate gold (nano-Au) monolayer as sensing platform has been described. The nano-Au monolayer was obtained through chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups facilitated the formation of nano-Au monolayer on the surface of CCPE. A sequential competitive immunoassay format was performed on the nano-Au monolayer supported by CCPE using S. japonicum antibody (SjAb) and SjAg as a model system. The assay comprised first loading of SjAb on nano-Au monolayer, then blocking in BSA solution, followed by a competitive incubation in the buffer containing the SjAg (analyte) and SjAg labeled with horseradish peroxidase (HRP) and finally the amperometric detection with hydroquinone as an enzymatic substrate. The dynamic concentration range for SjAg assay is 0.11-22.4&mu;gml^-1 with a detection limit of 0.06&mu;gml ^-1. The feasibility of regenerating nano-Au monolayer for consecutive assays was demonstrated by a simple chemical treatment after each determination. The simple manipulations of construction of nano-Au monolayer, low-cost and improved sensitivity are main features of proposed immunosensing method. &copy;2003 Elsevier B.V. All rights reserved.

语种： 英文

作者： Yang, Y;Lei, CX;Liu, ZM;Liu, YL;Shen, GL;Yu, RQ*

期刊： ANALYTICAL LETTERS,2004年37(11):2267-2282 ISSN：0003-2719

通讯作者： Yu, RQ

作者机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Univ, Dept Environm Chem & Engn, Yiyang, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： dopamine;ascorbic acid;beta-cyclodextrin polymer;carboxymethylated beta-cyclodextrin polymer;cyclic voltammetry;differential pulse voltammetry

摘要： A highly selective and sensitive electrochemical sensor modified with carboxymethylated, beta-cyclodextrin (CM-beta-CDP) polymer film was fabricated for the determination of dopamine (DA) in the presence of ascorbic acid (AA). The electrochemical behavior of DA and AA at the chemically modified electrode was investigated by cyclic voltammetry (CV) and differential pulse voltammetry. The response mechanism of CM-beta-CDP film for DA was based on the combination of electrostatic and inclusion interaction of CM-beta-CDP for DA, which was distinguished from the response mechanism of the charged polymer film modified electrode that only relied upon the electrostatic interaction between charged polymer film and DA (or AA) to realize the separation of overlapping peak potential of DA and AA. The CM-beta-CDP film showed preferable analytical performance characteristics in catalytic oxidation of DA compared with the beta-CDP polymer film. Under optimized conditions, it was feasible for the electrode modified with the CM-beta-CDP film to selectively determine DA in the presence of a large excess of AA. A linear calibration plot was obtained over the range 0.8-60 muM with a sensitivity of 65 nA/muM and a detection limit (signal/noise (S/N) = 3) of 0.2 muM in the presence of 1.0mM AA. This electrochemical sensor showed excellent sensitivity, repeatability, stability, and recovery for the determination of DA. The interference of AA with the determination of DA could be efficiently eliminated.

语种： 英文

作者： Lei, Cun-Xi;Wu, Jing;Wang, Hua;Shen, Guo-Li;Yu, Ru-Qin

期刊： Talanta,2004年63(2):469-474 ISSN：0039-9140

通讯作者： Yu, RQ

作者机构： [Yu, Ru-Qin] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Electrochemical immunoassay;Electrostatic interaction;Transferrin;Alginate;Chitosan-entrapped carbon paste electrode

摘要： A new electrochemical immunoassay strategy was proposed based on electrostatic interaction of natural polymers. The chitosan-entrapped carbon paste electrode (CCPE) was used as the base electrode, alginates as carriers for the reactants and horseradish peroxidase (HRP) as enzyme label in the immunoassay. The immunoassay was firstly carried out using the homogeneous competitive immune format and a very high rate of immune reaction was reached since both reacting antibodies and antigens were in a solution phase. Subsequently quick heterogeneous separation of immune complexes attached to the alginates molecule from the solution phase was realized by making use of the strongly electrostatic attraction between the protonated amino groups of chitosans on the surface of CCPE and carboxylic groups of alginates. After washing the surface of CCPE, amperometric detection with hydroquinone and hydrogen peroxide as enzymatic substrates was carried out. A new surface of CCPE for repeated use in another assay can be obtained by polishing the original used one. The feasibility of the above approach was demonstrated using transferrin and transferrin-antibody as a model system. The dynamic concentration range for transferrin assay was 1.9 to 78.8 &mu;g ml^-1. &copy;2003 Elsevier B.V. All rights reserved.

语种： 英文

作者： Wu, Jing;Lei, Cunxi;Yang, Haifeng;Wu, Xumei;Shen, Guoli;Yu, Ruqin

期刊： Sensors and Actuators, B: Chemical,2005年107(2):509-515 ISSN：0925-4005

通讯作者： Shen, GL

作者机构： [Shen, Guoli] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.

通讯机构： [Shen, GL] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

关键词： oxidized boron-doped diamond electrode;Vitamin B-6;ruthenium (III) tris(2,2 ')bipyridyl (Ru(bpy)(3)(3+))

摘要： The preparation and electrochemical characteristics of oxidized boron-doped diamond electrode modified by ruthenium tris(2,2&prime;)bipyridyl (Ru(bpy) <inf>3</inf>³⁺) are described. The Ru(bpy)<inf>3</inf> ³⁺-modified boron-doped diamond electrode has strong electrocatalytic effects on the redox of Vitamin B<inf>6</inf> (VB<inf>6</inf>). The oxidization and reduction peak potentials of VB<inf>6</inf> are 167.6 and -113 mV, respectively. In the presence of a large amount of Vitamins B<inf>1</inf> (VB<inf>1</inf>) and B<inf>2</inf> (VB<inf>2</inf>), the redox peak potentials of VB<inf>6</inf> shift slightly while VB<inf>1</inf> and VB<inf>2</inf> are not reduced or oxidized on the modified electrode. The catalytic mechanism and the effect of the film thickness were discussed. The influence of various experimental parameters on the Ru(bpy)<inf>3</inf>³⁺-modified boron-doped diamond electrode in VB<inf>6</inf> solution (i.e. frequency and amplitude of SWV, pH and scan rate) was investigated. A sensitive square wave voltammetric response for VB<inf>6</inf> is obtained covering a linear range from 0.2775 &mu;M to 0.3686 mM with a detection limit of 6.319 &times;10 ^-8 mol/L in the presence of a large amount of VB<inf>1</inf> and VB<inf>2</inf>. The modified electrode showed good stability and reproducibility with satisfactory recovery results. The determination of VB<inf>6</inf> in tablets using this modified electrode showed promising results compared with the traditional methods. &copy;2004 Elsevier B.V. All rights reserved.

语种： 英文

作者： Lei, Cun-Xi;Hu, Shun-Qin;Gao, Na;Shen, Guo-Li;Yu, Ru-Qin

期刊： Bioelectrochemistry,2004年65(1):33-39 ISSN：1567-5394

通讯作者： Yu, RQ

作者机构： [Yu, Ru-Qin] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.;Zhuzhou Teachers Coll, Dept Chem, Zhuzhou 412007, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Amperometric biosensor;Horseradish peroxidase;Hydrogen peroxide;Nano-Au monolayer

摘要： A novel strategy for fabricating horseradish peroxidase (HRP)-based H <inf>2</inf>O<inf>2</inf> sensor has been developed by combining the merits of carbon sol-gel supporting matrix and nano-scaled particulate gold (nano-Au) mediator. The thiol functional group-derived carbon ceramic electrode (CCE) was first constructed using (3-mercaptopropyl) trimethoxy silane as sol-gel monomer. Then, the stable nano-Au monolayer was obtained through covalent linkage between nano-Au and thiol group on the surface of CCE. The experimental results showed that nano-Au monolayer formed not only could steadily immobilize HRP but also efficiently retain its bioactivity. Hydrogen peroxide was detected with the aid of hydroquinone mediator to transfer electrons between the electrode and HRP. The process parameters for the fabrication of the enzyme electrode and various experimental variables such as the operating potential, mediator concentration and pH of background electrolyte were explored for optimum analytical performance of the enzyme electrode. The biosensor had a fast response of less than 8 s with linear range of 1.22&times;10^-5 to 1.10&times;10^-3mol l^-1 and a detection limit of 6.1&times;10^-6mol l^-1. The sensitivity of the sensor for H<inf>2</inf>O<inf>2</inf> was 0.29 A l mol^-1 cm^-2. The activation energy for enzyme reaction was calculated to be 10.1 kJ mol ^-1. The enzyme electrode retained 75% of its initial activity after 5 weeks storage in phosphate buffer at pH 7. &copy;2004 Elsevier B.V. All rights reserved.

语种： 英文

作者： Liu, Rong;Lei, Cunxi*;Zhong, Tongsheng;Long, Liping;Wu, Zhaoyang;Huan, Shuangyan;Zhang, Qi

期刊： Analytical Methods,2016年8(5):1120-1126 ISSN：1759-9660

通讯作者： Lei, Cunxi

作者机构： [Zhong, Tongsheng; Zhang, Qi; Lei, Cunxi; Liu, Rong; Long, Liping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Wu, Zhaoyang; Liu, Rong; Huan, Shuangyan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Lei, Cunxi] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.

摘要： A graphene (GN) and ionic liquid (IL) modified selenium-doped carbon paste electrode (GN/IL/Se/CPE) was established to simultaneously determine trace Cu(ii) and Sb(iii) by differential pulse voltammetry (DPV). Several parameters which affect the stripping current response were investigated and optimized, including the proportion of Se powder, concentration of hydrochloric acid, deposition time and deposition potential. The DPV curves were recorded by simultaneous deposition of Cu(ii) and Sb(iii) at -0.4 V for 50 s in a supporting electrolyte solution HCl of 0.30 mol L^-1. Under the optimized conditions, the GN/IL/Se/CPE sensor has a good linear response in the range of 2.0 &times;10^-6to 7.0 &times;10^-5mol L^-1for Cu(ii) and 2.0 &times;10^-6to 4.0 &times;10^-5mol L^-1for Sb(iii). The detection limits were 6.6 &times;10^-7mol L^-1for Cu(ii) and 4.3 &times;10^-7mol L^-1for Sb(iii). The sensor showed favorable stability and higher sensitivity. The present work extends the scope of these devices as mercury-free sensors and can be used to detect Cu(ii) and Sb(iii) simultaneously. &copy;The Royal Society of Chemistry 2016.

语种： 英文

作者： Lei, CX;Yang, Y;Wang, H;Shen, GL;Yu, RQ*

期刊： Analytica Chimica Acta,2004年513(2):379-384 ISSN：0003-2670

通讯作者： Yu, RQ

作者机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Univ, Dept Environm Chem & Engn, Yiyang 413049, Peoples R China.

通讯机构： [Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Amperometric immunosensor;Nano-Au monolayer;Carbon ceramic electrode;Complement III

摘要： An electrochemical immunosensor based on nano-size particulate gold (nano-Au) monolayer as sensing interface has been developed for probing complement III (C<inf>3</inf>). The thiol functional group-derived carbon ceramic electrode (CCE) was firstly constructed using (3-mercaptopropyl) trimethoxy silane (MPTMOS) as sol-gel monomer. The stable nano-Au monolayer was obtained resulting from covalent combination between nano-Au and thiol group on the surface of CCE. The nano-Au monolayer formed was utilized as a sensing platform for the immobilization of C<inf>3</inf> antibody (anti-C<inf>3</inf>) and subsequent immunoreaction. A competitive immunoassay format was adopted with horseradish peroxidase (HRP)-C<inf>3</inf> as a tracer, hydroquinone and hydrogen peroxide as the enzymatic substrates. The dynamic concentration range for C<inf>3</inf> is 0.08-5.6 &mu;g ml^-1. The feasibility of regenerating nano-Au monolayer for consecutive assays was demonstrated by a simple chemical treatment after each determination. The high stability of formed nano-Au monolayer, readily adsorptive immobilization of antibody on nano-Au monolayer, efficient activity retention of loading immunoreactants as well as the simple operation for the formation of nano-Au monolayer make proposed methodology an attractive alternative for the designing new-type immunosensors. &copy;2004 Elsevier B.V. All rights reserved.

语种： 英文

作者： Hu, SQ;Xie, ZM;Lei, CX;Shen, GL*;Yu, RQ

期刊： Sensors and Actuators, B: Chemical,2005年106(2):641-647 ISSN：0925-4005

通讯作者： Shen, GL

作者机构： [Shen, GL] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Zhu Zhou Teachers Coll, Dept Chem, Zhuzhou 412007, Peoples R China.;Hunan City Coll, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.

通讯机构： [Shen, GL] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.

关键词： Capacitive immunosensor;Gold nanoparticles;Oligomer layer;Transferrin

摘要： A new strategy for constructing the capacitance immunosensor was developed based on gold nanoparticles, which were employed as the platform to immobilize immunospecies. Gold nanoparticles were fixed on the gold electrode, on which the oligomer layer of o-aminobenzenthiol was pre-formed via electrochemical polymerization. An interesting immunospecies was loaded on gold nanoparticles to complete the fabrication of capacitance immunosensor. The changes in morphology of the gold electrode during the experiments were revealed by atomic force microscopy (AFM). The properties of the gold electrode were investigated by cyclic voltammetry, in the presence of an electro-active species as indictor. Also, potentiostatic pulse technique was used in order to evidence the changes of electrode capacitance. The feasibility of the proposed capacitive immunosensor was evaluated for a non-competitive heterogeneous immunoassay of transferrin. The assay covers a relatively large dynamic range of 0.125-100 ng/ml transferrin with a detection limit of 80 pg/ml and a relative high signal-to-noise ratio. &copy;2004 Elsevier B.V. All rights reserved.

语种： 英文

作者： Lin, Li*;Lei, Cunxi;Zhou, Jicheng

作者机构： [Lei, Cunxi; Lin, Li] Hunan City Univ, Dept Chem & Environm Engn, Hunan City 413000, Peoples R China.;[Zhou, Jicheng] Xiangtan Univ, Dept Chem Engn, Xiangtan 411105, Peoples R China.

会议名称： International Conference of Environment Materials and Environment Management

会议时间： JUL 24-25, 2010

会议地点： Harbin, PEOPLES R CHINA

会议主办单位： [Lin, Li;Lei, Cunxi] Hunan City Univ, Dept Chem & Environm Engn, Hunan City 413000, Peoples R China.^[Zhou, Jicheng] Xiangtan Univ, Dept Chem Engn, Xiangtan 411105, Peoples R China.

会议论文集名称： Advanced Materials Research

关键词： neutralization hydrolysis;TiO2 nanomaterial;photocatalytic

摘要： A neutralization hydrolysis route has been used to synthesize titanium oxide nanoparticles. Transmission electron microscopy(TEM) and X-ray diffraction(XRD) characterization results showed the TiO2 nanoparticles were single anatase phases when calcined at 600 degrees C-800 degrees C for 2h. The particle diameter ranged from 15 to 30nm. The pH value was key factor for precursor composition. When pH value 2-4, the H2TiO3 precursor with low agglomeration was obtained. As-prepared anatase TiO2 nanoparticles, during photodegradation of methylic orange under UV-light irradiation(lambda=254nm), exhibited excellent activity, more than 95.5% methylic orange was degraded in 1h.

语种： 英文

作者： Liu Rong;Long Li-Ping*;Lei Cun-Xi;Wu Zhao-Yang;Liu Yu;Li Si-Jia

期刊： 分析化学,2016年44(3):385-390 ISSN：0253-3820

通讯作者： Long Li-Ping

作者机构： [Long Li-Ping; Liu Rong; Liu Yu; Lei Cun-Xi; Li Si-Jia] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Liu Rong; Wu Zhao-Yang] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

通讯机构： [Long Li-Ping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.

关键词： 分子印迹;桑色素;电化学传感器;电聚合

摘要： 以邻氨基酚(o-AP)为功能单体,桑色素为模板分子,基于分子间的相互作用力,在金电极表面电聚合制备具有特异性识别孔穴的桑色素分子印迹传感器膜。采用循环伏安法(CV)、差分脉冲伏安法(DPV)等研究了分子印迹膜的性能和分子印迹效应。探索了聚合膜配比及聚合扫描圈数对传感器性能的影响,优化了洗脱时间和印迹时间。比较了此传感器对其结构相似物的选择性响应,发现其对桑色素检测具有良好的选择性。在最佳实验条件下,此传感器对桑色素浓度定量测定范围为0.05 ~ 1.70 μmol /L,线性方程为I(μA)= 1.0800lgc(mol /L)+ 9.3599,R= 0.9934,检出限为0.01 μmol /L。用此传感器测定黑茶样品中桑色素的含量,加标回收率为104.0% ~ 108.0%。

语种： 中文

作者： 胡拥军;雷存喜;方芳

期刊： 化工时刊,2015年(5):36-38 ISSN：1002-154X

作者机构： 湖南城市学院化学与环境工程学院,湖南 益阳,413000;[胡拥军; 雷存喜; 方芳] 湖南城市学院

关键词： 校企合作;毕业设计(论文);质量监控;地方院校

摘要： 分析了当前地方本科院校毕业设计(论文)教学工作存在的问题及校企合作现状,研究了校企合作人才培养模式下开展本科毕业设计(论文)的意义,提出从企业资质、选题质量、学生设计和研究过程等评价指标,企业导师、高校、学生等多元评价主体,对校企合作模式下的毕业设计(论文)教学进行质量监控。

语种： 中文

作者： 唐赤;段敏;雷存喜

期刊： 广东化工,2011年38(5):207-208,204 ISSN：1007-1865

作者机构： 湖南城市学院,化学与环境工程系,湖南,益阳,413000;湖南箴言中学,湖南,益阳,413046;[段敏] 段敏;[雷存喜] 雷存喜;[唐赤] 唐赤

关键词： 微波消解;原子吸收光谱法;葛粉;铁;钙;铜;锰

摘要： 建立了用微波消解法处理样品、火焰原子吸收光谱法测定葛粉中Fe、Ca、Cu、Mn元素含最的方法,优化了微波消解、原子吸收测定的工作条件.建议的方法测得葛粉中Fe、Ca、Cu、Mn的含量分别为:27.83 μg·g<'-1>、272.46 μg·g<'-1>、6.98μg·g<'-1>、9.45 μg·g<'-1>,加标同收率在96.9%103.1%之间.建议的方法可用于实际葛粉样品中相应金属元素含量测定.

语种： 中文

作者： 刘石泉;赵运林;胡治远;雷存喜

期刊： 茶叶,2011年37(4):225-230 ISSN：0577-8921

作者机构： 湖南农业大学生物科学与技术学院,湖南长沙410128／湖南城市学院化学与环境工程系,湖南益阳413000／黑茶研究所,湖南益阳413000;湖南农业大学生物科学与技术学院,湖南长沙410128／湖南城市学院化学与环境工程系,湖南益阳413000;湖南农业大学生物科学与技术学院,湖南长沙,410128;湖南城市学院化学与环境工程系,湖南益阳413000／黑茶研究所,湖南益阳413000;[刘石泉; 赵运林; 胡治远] 湖南农业大学

关键词： 冠状散囊菌;溶菌酶;纤维素酶;果胶酶;CTAB法;SDS法;DNA提取

摘要： 为快速、简单、有效的获得冠状散囊菌DNA，分别使用溶菌酶破壁法、纤维素酶破壁法、果胶酶破壁法、CTAB法、SDS法提取冠状散囊菌菌丝体和子囊孢子的基因组DNA，然后进行琼脂糖凝胶电泳、ITS序列的PCR扩增检测以及紫外分光光度计测定DNA浓度和纯度。实验结果表明：5种方法均能提取出冠状散囊菌基因组DNA，酶法中溶菌酶最好、果胶酶次之、纤维素酶较差，但三者差异不显著；SDS法明显优于CTAB法，且在五种方法中效果也是最好的，DNA浓度可达1．69mg／mL；五种方法都证实用冠状散囊菌菌丝体作为DNA提取材料优于用子囊孢子。

语种： 中文

作者： 俞福君;雷存喜;袁华斌

期刊： 未来英才,2014年(7):109-110

作者机构： [俞福君; 雷存喜; 袁华斌] 湖南省益阳湖南城市学院

关键词： 执业能力;新建本科院校;应用型;实践教学;质量监控

摘要： 提高学生执业能力是应用型本科院校人才培养的首要任务，而实践教学是培养学生创新精神、实践能力和就业竞争力的有效途径。目前新建应用型本科院校的实践教学质量监控体系存在诸多问题。鉴于此，新建应用型本科院校应重视学生实践能力培养，加大教学改革力度，明确监控实施主体，健全实践教学评价标准，注重实践过程监控，架构实践教学质噩监控新体系。

语种： 中文

作者： 汤青云;钟桐生;龙立平;雷存喜;谢志美

期刊： 化学教育,2002年23(11):38 ISSN：1003-3807

作者机构： [汤青云; 钟桐生; 龙立平; 雷存喜; 谢志美] 湖南城市学院化学系;湖南城市学院化学系 湖南益阳413049

关键词： 乙醚;实验仪器;有机化学实验;复旦大学化学系;东北师范大学;实验教材;师范院校;兰州大学;化学化工;大专院校;组编;上海;教研;江苏;华南;广西

摘要： 1引言 华南师范学院、东北师范大学、上海师范学院、江苏师范学院、广西师范学院合编,曾昭琼主编的<有机化学实验>[1]及兰州大学、复旦大学化学系有机化学教研组编写的<有机化学实验>[2],是我国大专院校,特别是师范院校化学化工系使用最为普遍的有机化学实验教材.

语种： 中文

作者： 雷存喜;曾冬铭;舒万艮;刘又年

期刊： 稀有金属,1999年23(2):100-103 ISSN：0258-7076

作者机构： [雷存喜] 湖南益阳师范专科学校化学系;[曾冬铭; 舒万艮; 刘又年] 中南工业大学化学系

关键词： 含铟浸出液;铟与锑、铁分离;铟富集

摘要： 研究了从冶炼锑渣浸出液中有效地分离铟与锑、铁的工艺条件 ;锑渣浸出液经P2 0 4 煤油溶液萃取、草酸溶液洗涤有机相和稀盐酸反萃取 ,可制得纯度 90 %以上的三氯化铟溶液 ;利用稀盐酸循环反萃取 ,使铟富集 ,浓度达到 2 5～ 30g/L。

语种： 中文

作者： 雷存喜;文瑞明;俞善信

期刊： 湘潭大学自然科学学报,2002年24(1):57-59 ISSN：1000-5900

作者机构： [雷存喜; 文瑞明; 俞善信] 益阳师范高等专科学校化学系;益阳师范高等专科学校化学系 湖南益阳413049

关键词： 氯乙酸酯;合成;硫酸氢钠;催化;表征

摘要： 报道了在一水硫酸氢钠存在下，氯乙酸和不同醇的酯化作用。硫酸氢钠能够催化合成氯乙酸酯，且随着醇的分子量增大，产品的收率提高；一级醇比二级醇易于酯化，三级醇由于空间位阻，反应很难进行；利用硫酸氢钠催化二级醇，其分水量大大超过理论分水量；对于易溶于水的醇的酯化，在回流过程中，有的发生温度稍有下降现象；为了提高其产品收率，反应必须在有带水剂的情况下进行。研究了催化剂的重复使用性能，并利用折射率、元素分析及红外光谱对丁醇、戊醇和环已醇的氯乙酸酯进行了表征。

语种： 中文