A new sol-gel-derived silicate/alginate inorganic-organic hybrid film was developed when the alginate was used as additive. The resulting composite film was applied to entrap tyrosinase for fabricating phenol biosensor. Firstly, a homogeneous stock methyltrimethoxysilane (MTMOS) /alginate was prepared by using MTMOS as the precursor and alginate as the additive. 0.3 mL of methanol, 0.2 mL of MTMOS. 0.2 mL of 10 g/L sodium alginate, and 0.2 mL of 0.01 mol/L HCl were mixed in a small weigh vial at room temperature and then sonicated for approximately 20 min to obtain a uniform soil. Sequently, 2...
