茯砖茶中冠突散囊菌的次级代谢产物及其生物活性研究
作者：
彭晓赟;梁法亮;李冬利;陈玉婵;陶美华;...
期刊：
中草药 ,2013年44(14):1881-1886 ISSN：0253-2670
通讯作者：
Zhao, Y.-L.
作者机构：
[陶美华; 梁法亮; 陈玉婵; 章卫民; 李冬利] Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China;[赵运林] Dark Tea Institute of Yiyang City, Department of Chemistry and Environment Engineering, Hunan City College, Yiyang 413000, China;[彭晓赟] Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China, Dark Tea Institute of Yiyang City, Department of Chemistry and Environment Engineering, Hunan City College, Yiyang 413000, China
通讯机构：
[Zhao, Y.-L.] D;Dark Tea Institute of Yiyang City, , Yiyang 413000, China
关键词：
茯砖茶;冠突散囊菌;次级代谢产物;抗菌活性;细胞毒活性;苯甲醛;苔黑酚
摘要：
目的研究茯砖茶中冠突散囊菌Eurotium cristatum的次级代谢产物及其生物活性。方法采用正、反相硅胶柱、凝胶柱和薄层制备等色谱方法和重结晶技术进行分离纯化,通过各种波谱技术进行结构鉴定;以3种肿瘤细胞SF-268、MCF-7和NCI-H460为供试细胞株,采用SRB法对化合物进行细胞毒活性研究;以金黄色葡萄球菌、大肠杆菌、肠炎沙门氏菌肠炎亚种、痢疾志贺氏菌和普通变形杆菌等肠道致病菌为供试细菌,采用MTT法对化合物进行抗菌活性研究。结果从茯砖茶中冠突散囊菌发酵液提取物中分离得到9个化合物,分别鉴定为大黄素甲醚（1）、1,5-二羟基-3-甲氧基-7-甲基蒽醌（2）、灰绿曲霉黄色素（3）、2-（2′,3-环氧基-1′,3′-庚二烯基）-6-羟基-5-（3-甲基-2-丁烯基）苯甲醛（4）、2-（2′,3-环氧基-1′,3′5′-庚三烯基）-6-羟基-5-（3-甲基-2-丁烯基）苯甲醛（5）、2-（3E,5E-庚二烯基）-3,6-二羟基-5-（3-甲基-2-丁烯基）苯甲醛（6）、酪醇（7）、对羟基苯甲酸（8）、苔黑酚（9）。活性测试结果显示化合物3～6对3种肿瘤细胞株有较好的细胞毒活性;除化合物1和6外,其他化合物对普通变形杆菌具有明显的抑制活性,其中化合物9还对金黄色葡萄球菌和大肠杆菌表现出很好的抑菌效果。结论苯甲醛类化合物是冠突散囊菌的主要代谢产物,化合物7和9为首次从该属真菌中分离得到,化合物2、3、5、6和8均为首次从该真菌中分离得到。
语种：
中文
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Novel pyridine Schiff base-manganese(ii) complexes: Synthesis, characterization and catalytic reactivity of the epoxidation of cyclohexene
作者：
Li, Zhongying;Liu, Changhui;Tang, Ruiren* ;Liu, Guiyin
期刊：
RSC Advances ,2013年3(25):9745-9751 ISSN：2046-2069
通讯作者：
Tang, Ruiren
作者机构：
[Li, Zhongying; Tang, Ruiren; Liu, Guiyin] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Tang, Ruiren] C;Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
摘要：
Four novel pyridine Schiff bases derived from 2,6-pyridinedicarbaldehyde and substituted o-hydroxyl-aromatic amines and their manganese(ii) complexes were synthesized and characterized by elemental analyses, ESI-MS analysis, thermal analysis (TGA), H-NMR, IR and UV-vis. The activity of these complexes as catalysts in cyclohexene autoxidation were investigated and the effects of various reaction conditions on the catalytic reaction were optimized to obtain as high as 99.6% conversion with 95.0% selectivity of epoxycyclohexane at 40°C for 6 h using molecular oxygen as an oxidant in acetonitrile. The result presents excellent catalysts for epoxidation of cyclohexene under mild temperature and atmospheric oxygen (O<inf>2</inf>) pressure. ©2013 The Royal Society of Chemistry.
语种：
英文
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Preparation and characterization of poly(amic acid)-stabilized silver nanoparticles
作者：
Li Jun;Fang Yue-cheng;He Guo-wen;Li Heng-feng*
期刊：
中南大学学报(英文版) ,2013年20(6):1475-1481 ISSN：2095-2899
通讯作者：
Li Heng-feng
作者机构：
[Fang Yue-cheng; He Guo-wen; Li Heng-feng; Li Jun] Cent South Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China.;[He Guo-wen] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Li Heng-feng] C;Cent South Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
Diffusion controlled growth;Heterogeneous nucleation;Poly(amic acid);Polycrystalline state;Reaction mixture;Reduction of silver nitrates;Silver nanoparticles;Spherical morphologies;Crystallization kinetics;Nanoparticles;Nitrogen compounds;Ultraviolet visible spectroscopy;Silver
摘要：
Stable and monodispersed silver nanoparticles were produced through a mild, convenient, one-pot method based on the reduction of silver nitrate in the presence of poly(amic acid) (PAA) as a stabilizer. The surface plasma band transition was monitored along with time in the reaction mixture for three sets of experiments by ultraviolet-visible spectroscopy. Analysis of the data with the Avrami equation yielded n exponent with values between 0.5 and 1.5, demonstrating three-dimensional heterogeneous nucleation and diffusion-controlled growth, accompanied by soft impingement effect. XRD and TEM analyses show a softly agglomerated polycrystalline state and a nearly spherical morphology (<50 nm) of nanoparticles. The FT-IR result indicates that the PAA molecular structure could be hardly influenced by the formation of nanoparticles. © 2013 Central South University Press and Springer-Verlag Berlin Heidelberg.
语种：
英文
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Study of dark tea residue's high efficiency absorption in treatment of Cr-bearing waste water
作者：
Song, Juanjuan
期刊：
Journal of Chemical and Pharmaceutical Research ,2013年5(11):802-810 ISSN：0975-7384
通讯作者：
Song, J.
作者机构：
[Song, Juanjuan] School of Chemical and Environmental Engineering, Hunan City University, Yiyang, Hunan, China
关键词：
Absorption dynamics;Adsorption kinetics;Dark tea;Development prospects;Initial concentration;Langmuir adsorption isotherms;Monolayer adsorption;Pseudo-second-order equations;Absorption;Chromium compounds;Heavy metals;Metal ions;pH;Adsorption;chromium;adsorption;adsorption kinetics;article;heavy metal removal;mathematical model;particle size;pH;tea;waste water recycling
摘要：
As an absorbent dark tea residue can get rid of heavy metal ion effectually and do no harm to the environment. From the dynamic experiment on dark tea residue's absorption of Cr(VI), the absorption dynamic curve and absorption isothermal curve are obtained. The effects of pH value, initial concentration, dosage of residue, adsorption time and other factors on absorption are studied and the result shows that: when the pH value of the waste water is 2, the initial concentration is 100mg/L, the adsorption time is 60min, the dosage of dark tea residue is 1g, the maximum adsorption rate reaches 99.96%.The adsorption conforms with the Langmuir adsorption isotherm, it is a kind of monolayer adsorption, the adsorption kinetics curve conforms with pseudo-second-order equation. In the end discusses the development prospect of dark tea residue.
语种：
英文
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茯砖茶中碘含量的测定
作者：
刘蓉;龙立平;钟桐生;赵运林;刘小英;...
期刊：
广州化工 ,2013年41(15):121-123 ISSN：1001-9677
作者机构：
湖南城市学院化学与环境工学院,湖南 益阳,413000;[龙立平; 刘蓉; 陈艳; 钟桐生; 刘小英; 赵运林] 湖南城市学院
关键词：
茯砖茶;碘;间接碘量法
摘要：
研究了茯砖茶中碘含量的测定方法及方法的操作条件.将茶样在560℃温度下灰化后,用间接碘量法测定茶样中碘的含量.以KMnO4为氧化剂,在碱性条件下,茶样中的I-被KMnO4氧化为IO3-,并在酸性条件下加入KI与IO3-反应生成I2,用Na2S2O3对I2进行滴定,根据Na2S2O3的消耗量得到茶叶中的碘含量.
语种：
中文
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Highly selective fluorescence turn-on sensor for hydrogen sulfide and imaging in living cells
作者：
Wang, Jiaoliang;Long, Liping* ;Xie, Dan;Zhan, Youwen
期刊：
Journal of Luminescence ,2013年139:40-46 ISSN：0022-2313
通讯作者：
Long, Liping
作者机构：
[Wang, Jiaoliang; Xie, Dan; Zhan, Youwen; Long, Liping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.
通讯机构：
[Long, Liping] H;Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.
关键词：
Cell imaging;Chemsensor;CuS;Detection limits;Displacement method;Fluorescence intensities;Fluorescent response;Selective fluorescence;Dynamic response;Fluorescence;Hydrogen sulfide;Negative ions;Sulfur compounds;Sulfur determination;Copper compounds
摘要：
A displacement method of detecting hydrogen sulfide in aqueous media based on complex L-Cu ensemble is developed. Once combined with Cu2+, complex L-Cu displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induce the revival of fluoresecence of compound L, which is quenched by Cu2+, resulting in turn-on type sensing sulfide anion. Complex L-Cu exhibits a highly sensitive fluorescent response toward S2- by releasing compound L to give a remarkable change with 20-fold fluorescence intensity enhancement under 2 equivalent of S2- added in Tris-HCl/ DMF (20 mM, 6/4, v/v), and also exhibits a dynamic response range for S2- from 5×10-7 to 5×10-6 M, with a detection limit of 0.18 μM. In addition, the turn-on fluorescent change upon the addition of S2- is also applied in cell imaging.© 2013 PublishedbyElsevierB.V.
语种：
英文
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嵌段中孔材料SBA-15(SH)的制备及应用
作者：
Zhou Le-Zhou* ;He Guo-Wen;Fu Sheng;Gao Shou-Quan;Yu Ke-Ping
期刊：
分析化学 ,2013年41(7):1080-1085 ISSN：0253-3820
通讯作者：
Zhou Le-Zhou
作者机构：
[Fu Sheng; Yu Ke-Ping; Zhou Le-Zhou; Gao Shou-Quan] Hunan Prevent & Treatment Inst Occupat Dis, Changsha 410007, Hunan, Peoples R China.;[He Guo-Wen] Hunan City Univ, Chem & Environm Engn Coll, Yiyang 413000, Peoples R China.
通讯机构：
[Zhou Le-Zhou] H;Hunan Prevent & Treatment Inst Occupat Dis, Changsha 410007, Hunan, Peoples R China.
关键词：
嵌段中孔材料SBA-15(SH);重金属离子
摘要：
采用共表面活性剂法和嫁接法合成了孔道结构高度有序、粒径均匀且孔径较大的嵌段中孔材料SBA-15(SH),通过N_2吸附脱附实验测得材料的孔径为7.8 nm,比表面积为629 m~2/g,孔容为1.32 cm~3/g,核磁共振与红外光谱结果显示巯基的覆盖率达65%。探讨了嵌段中孔材料SBA-15(SH)动态吸附重金属离子的原理和最佳条件。在pH=7.5,常温下,Hg~(2+),Cd~(2+),Pb~(2+),Ag~+,Cr~(3+),Cr~(6+),Cu~(2+),Mn~(2+)和Zn~(2+)可被该材料定量吸附,动态吸附容量分别为17.1,18.7,22.6,12.7,9.7,10.8,10.8,19.2和15.5 mg/g,吸附的重金属离子可用6 mol/L HCl-2 g/L硫脲洗脱,采用原子荧光和原子吸收法测定过柱前后溶液及洗脱液中重金属离子的含量,加标回收实验显示回收率在89.2%～109.6%之间。本方法用于环境水样的处理和测定,结果满意。
语种：
中文
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Synthesis and Characterization of Fluorinated Polyimide/Silica Nanocomposites with Low Water Absorption and Dielectric Constant
作者：
He, Guowen;Li, Hengfeng* ;Chen, Jiangcong;Chen, Jun;Wen, Jiebin
期刊：
Asian Journal of Chemistry ,2013年25(1):139-143 ISSN：0970-7077
通讯作者：
Li, Hengfeng
作者机构：
[Chen, Jiangcong; Wen, Jiebin; Chen, Jun; Li, Hengfeng; He, Guowen] Cent S Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China.;[He, Guowen] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Li, Hengfeng] C;Cent S Univ, Sch Mat Sci & Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
Dielectric properties;Nanosilica;Polyimides;Water absorption
摘要：
A fluorine-containing polyimide(FPI) was synthesized by reacting 4,4'-(hexafluoroisopropylidene) diphthalic anhydride (6FDA) with 2,2'-bis-(trifluoromethyl)-4,4'-diaminobiphenyl (TFDB). A series of polyimide composite films with various contents of nanosilica were prepared by an in situ polymerization technique. The obtained composite materials were analyzed with FTIR and the results showed that the amino groups were completely imidized. The morphology of the composites was characterized with SEM and the results indicated that the nanosilica particles integral dispersed but micro-aggregated in the fluoro-contained polyimide matrix if the nanosilica content excessed 10 wt %. The nanocomposite films were found to hold comparable thermal stability with the pure fluoro-contained polyimide film. The humidity absorption rate decreased with the increase of silica content. The dielectric constant of fluoro-contained polyimide/ nanosilica films decreased with increasing the frequency and exhibited an arch curve profile with its maximum value of less than 3, indicating the excellent dielectric property.
语种：
英文
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利用轮藻修复白洋淀富营养化水体研究
作者：
李必才;黄彩红;孟睿;宋娟娟;彭晓赟;...
期刊：
湿地科学 ,2013年11(3):383-387 ISSN：1672-5948
作者机构：
[李必才; 宋娟娟; 彭晓赟] 湖南城市学院化学与环境工程学院;[黄彩红; 孟睿; 何连生] 中国环境科学研究院;[李钢] 保定市排水总公司
关键词：
轮藻;叶绿素a;营养盐;白洋淀
摘要：
选取白洋淀优势沉水植物轮藻(Chara sp.)作为建群种,研究其修复白洋淀富营养化水体的效果。室内模拟实验表明,轮藻群落对水体叶绿素a和营养盐的抑制效果明显,实验结束时,水体叶绿素a、氨氮、总氮和总磷含量分别为实验开始时的24.85%、39.2%、30.7%和23.2%。野外不同轮藻覆盖度围隔实验结果表明,围隔中轮藻覆盖度为80%时,水体中叶绿素a、氨氮、磷酸盐和总磷含量减少最明显。实验结果表明,轮藻作为建种群修复白洋淀富营养化水体是可行的。
语种：
中文
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Study on Adsorption Properties of Sb3+by Modified Diatomite
作者：
Song, Juanjuan* ;Tu, Li
作者机构：
[Song, Juanjuan] Hunan City Univ, Sch Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.;[Tu, Li] Hunan City Univ, Sch Informat Sci & Engn, Yiyang 413000, Hunan, Peoples R China.
会议名称：
2012 International Conference on Ecology,Waste Recycling,and Environment(ICEWE 2012)
会议时间：
2012-04-01
会议地点：
Macao,China
关键词：
modified diatomite;stibium;adsorption
摘要：
With increasing the degree of state environmental protection, the comprehensive treatment of waste water containing stibium has become a new subject in stibium industry.In this study,we modified diatomite with calcium carbonate,and adsorptived waste water containing stibium using these modified diatomite.Then we explored the quality of modified diatomite,initial concentration,initial pH value,temperature and adsorpting time on adsorption. The adsorption isotherm of stibium fits Langmuir equation.The result shows that the modified diatomite has good adsorption on waste water containing stibium.
语种：
英文
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采用对甲苯磺酸催化研磨法合成苯甲醛缩苯胺衍生物
作者：
文瑞明;朱云辉;游沛清
期刊：
中南大学学报(自然科学版) ,2012年43(4):1249-1253 ISSN：1672-7207
通讯作者：
Wen, R.-M.(wenruiming@sohu.com)
作者机构：
[文瑞明; 朱云辉; 游沛清] Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, China
通讯机构：
Department of Chemistry and Environmental Engineering, Hunan City University, China
关键词：
苯甲醛缩苯胺;Schiff碱;研磨;对甲苯磺酸;缩合反应
摘要：
在对甲苯磺酸催化下,将芳香醛与芳胺于室温下研磨可得较高收率的苯甲醛缩苯胺衍生物,通过单因素实验研究研磨时间、对甲苯磺酸用量及取代基类型对缩合反应的影响。研究结果表明采用对甲苯磺酸催化研磨法合成苯甲醛缩苯胺衍生物最佳工艺条件是：芳香醛、芳胺、对甲苯磺酸的摩尔比为100：100：3,反应温度为室温,研磨时间为1~3 min,在此条件下,反应收率可达86.5%~95.5%,比采用溶剂回流法和常规研磨法所得收率有显著提高,而且反应时间减少0.5%;芳醛环上取代基对反应无影响,但芳胺环上邻、对位的强吸电子基抑制反应的进行;与传统方法相比,对甲苯磺酸催化研磨法具有反应时间短、条件温和、操作简单、收率高等优点,为同类化合物的合成提供了一个简便而有效的方法。
语种：
中文
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聚酰亚胺/二氧化硅-二氧化钛三元纳米复合材料的制备及介电性能
作者：
陈江聪;贺国文;陈俊;文杰斌;李衡峰
期刊：
高分子材料科学与工程 ,2012年28(11):153-157 ISSN：1000-7555
通讯作者：
Li, H.(lihengfeng@gmail.com)
作者机构：
[陈俊; 文杰斌; Chen, Jun; 李衡峰] School of Materials Science and Engineering, Central South University, Changsha 410083, China;[贺国文] Department of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China
通讯机构：
[Li, H.] S;School of Materials Science and Engineering, , Changsha 410083, China
关键词：
聚酰亚胺;纳米二氧化硅;纳米二氧化钛;介电常数;纳米复合材料
摘要：
以4，4’-二氨基二苯醚（ODA）和3，3，4，4’-二苯甲酮四酸二酐（BTDA）为单体，以正硅酸乙酯和钛酸丁酯为SiO2和TiO2的前驱体，采用溶胶-凝胶原位生成法制备了聚酰亚胺（PI）复合薄膜。采用红外光谱（FT—IR）、扫描电镜（SEM）、x射线衍射（XRD）和紫外分光光度计（UV—Vis）对复合材料进行结构表征和性能测试。结果表明，复合膜的热亚胺化反应完全；纳米SiO2、TiO2均匀分散于PI基体中；复合膜透明性好，对紫外光吸收较好。热重分析（TG）和介电性能测试表明，得到了一种热稳定性好、介电性能优良的复合材料，能够满足其在微电子器件领域的使用要求。
语种：
中文
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Ni-Pt/γ-Al_2O_3催化剂用于甲基环己烷催化脱氢的研究
作者：
王锋;杨运泉;王威燕
期刊：
燃料化学学报 ,2012年40(9):1128-1134 ISSN：0253-2409
通讯作者：
Yang, Y.-Q.(yangyunquan@xtu.edu.cn)
作者机构：
[王威燕; 王锋; 杨运泉] College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China;[王锋] College of Chemical and Environmental Engineering, Hunan City University, Yiyang 413000, China
关键词：
甲基环己烷;脱氢反应;Ni-Pt双金属催化剂;γ-氧化铝;纳米膜
摘要：
采用自制的比表面积为349 m2/g的纳米膜γ-Al2O3为载体,用等体积浸渍法制备了Ni-Pt/γ-Al2O3催化剂,采用比表面积测定、X射线衍射、扫描电镜和透射电镜等手段对所制备的催化剂进行了表征。利用微型连续管式反应器与气相色谱联用装置,考察了Ni-Pt/γ-Al2O3催化剂对甲基环己烷（MCH）气相脱氢的催化性能。结果表明,使用20%Ni-0.5%Pt/γ-Al2O3催化剂,在反应温度350℃,混合进样体积空速252 h-1条件下,甲苯转化率达到96.99%,选择性接近100%。
语种：
中文
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Homology Modeling of Mosquitocidal Cry30Ca2 of Bacillus thuringiensis and Its Molecular Docking with N-acetylgalactosamine
作者：
Zhao Xin Min;Zhou Pan Deng;Xia Li Qui*
期刊：
生物医学与环境科学(英文) ,2012年25(5):590-596 ISSN：0895-3988
通讯作者：
Xia Li Qui
作者机构：
[Xia Li Qui; Zhao Xin Min] Hunan Normal Univ, Coll Life Sci, Key Lab Microbial Mol Biol Hunan Prov, Changsha 410081, Hunan, Peoples R China.;[Zhao Xin Min; Zhou Pan Deng] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Xia Li Qui] H;Hunan Normal Univ, Coll Life Sci, Key Lab Microbial Mol Biol Hunan Prov, Changsha 410081, Hunan, Peoples R China.
关键词：
Cry30Ca2;Bacillus thuringiensis;Homology modeling;Mosquitoes;Molecular docking
摘要：
Objective: To investigate the theoretical model of the three-dimensional structure of mosquitocidal Cry30Ca2 and its molecular docking with N-acetylgalactosamine. Methods: The theoretical model of Cry30Ca2 was predicted by homology modeling on the structure of the Cry4Ba. Docking studies were performed to investigate the interaction of Cry30Ca2 with N-acetylgalactosamine on the putative receptor. Results: Cry30Ca2 toxin is a rather compact molecule composed of three distinct domains and has approximate overall dimensions of 95 by 75 by 60Å. Domain II is a helix bundle, Domain II consists of three antiparallel β-sheets, Domain III is composed of two β-sheets that adopt a β-sandwich fold. Residue 321Ile in loop1, residues 342Gln 343Thr and 345Gln in loop2, residue 393Tyr in loop3 of Cry30Ca2 are responsible for the interactions with GalNAc via 7 hydrogen bonds, 6 of them were related to the oxygen atoms of hydroxyls of the ligand, and one to the nitrogen of the ligand. Conclusion: The 3D structure of Cry30Ca2 resembles the previously reported Cry toxin structures but shows still some distinctions. Several residues in the loops of the apex of domain II are responsible for the interactions with N-acetylgalactosamine. © 2012 The Editorial Board of Biomedical and Environmental Sciences.
语种：
英文
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富金花茯散茶对高尿酸血症小鼠的影响
作者：
彭雨轩;熊哲;胡志远;曹忠良
期刊：
湖南城市学院学报(自然科学版) ,2012年(4):58-60 ISSN：1672-7304
作者机构：
湖南农业大学 园艺园林学院,长沙 410128;湖南城市学院 黑茶研究所,湖南 益阳 413000;[彭雨轩; 熊哲; 胡志远; 曹忠良] 湖南农业大学
关键词：
富金花茯散茶;高尿酸血症;尿酸;黄嘌呤氧化酶;腺苷脱氨酶
摘要：
研究富金花茯散茶对氧嗪酸钾致高尿酸血症小鼠的尿酸代谢与相关酶活性的影响.将60只雄性KM种小鼠随机分为空白组、模型组、阳性药组(别嘌呤醇)、茯茶低剂量组(125 mg/kg)、茯茶中剂量组(250 mg/kg)、茯茶高剂量组(500 mg/kg).连续灌胃7 d后腹腔注射氧嗪酸钾(300 mg/kg)造模,空白组注射等量蒸馏水.采样后测定小鼠血尿酸(UA)、肝脏黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)活性.造模后,茯茶高剂量组血尿酸浓度较对照组降低35.2%(P<0.05),肝脏XOD及ADA活性抑制率显著,XOD活性降低38%(P<0.05), ADA活性降低53.8%(P<0.05).富金花茯散茶对氧嗪酸钾导致的高尿酸血症有明显的改善作用,其作用机制与其抑制肝脏XOD、ADA活性有关.
语种：
中文
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三组分铜铬催化剂催化甘油制备羟基丙酮的研究
作者：
谢丹;李浔;龙立平;肖谷清;林立
期刊：
广州化工 ,2012年40(12):93-95,126 ISSN：1001-9677
作者机构：
湖南城市学院化学与环境学院,湖南益阳,413000;长沙理工大学化学与生物工程学院,湖南长沙,410076;[李浔] 长沙理工大学;[谢丹; 龙立平; 林立; 肖谷清] 湖南城市学院
关键词：
生物质甘油;Cu-Cr-Zn催化剂;羟基丙酮
摘要：
用共沉淀法制备了Cu-Cr-Zn催化剂,考察了Cu/Cr/Zn比、沉淀温度、沉淀时pH值、煅烧温度等制备条件对甘油制备羟基丙酮的影响,并对催化剂进行了表征。结果表明：共沉淀法制备Cu-Cr-Zn催化剂具有较高的催化活性,而且其稳定性和再生能力较好,适宜催化剂制备条件为：Cu/Cr/Zn比为1∶1∶0.5,沉淀温度为70℃,沉淀时pH值为8.0,煅烧温度为400℃,羟基丙酮的选择性可达到90.2%。
语种：
中文
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微生物在现代农业中的应用研究进展
作者：
龚亚辉
期刊：
现代农业科技 ,2012年(23):225-225,227 ISSN：1007-5739
作者机构：
湖南城市学院化学与环境学院,湖南益阳,413000;[龚亚辉] 湖南城市学院
关键词：
微生物;现代农业;应用
摘要：
介绍了3类微生物农药在植保方‘面的应用研究进展，并从茶叶领域、农业环保、现代农业领域等方面总结了微生物的应用，并提出微生物在其他领域的一些应用前景，以期为微生物在现代农业中的应用提供参考。
语种：
中文
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HPLC法测定茯砖茶中芦丁含量
作者：
刘蓉;雷存喜;赵运林;刘石泉;陈丹
期刊：
北京农业 ,2012年(09):4-5 ISSN：1000-6966
作者机构：
湖南城市学院化学与环境工学院,湖南益阳,413000;[刘蓉; 刘石泉; 赵运林; 雷存喜; 陈丹] 湖南城市学院
关键词：
茯砖茶;芦丁
摘要：
建立了茯砖茶中主要黄酮类物质芦丁的高效液相色谱测定方法.采用Hypersil ODS色谱柱C18色谱柱,流动相:A乙腈；B磷酸溶液(pH=2.5),梯度洗脱,流速1.0 mL/min,检测波长258 nm,样量为20 μL,柱温30℃.对照品芦丁的回归方程Y=16 007.7 ρB(μg/mL)-11 115.2,R=0.999 3,线性范围为10.000-80.000 mg/L,平均回收率99.010 0％,RSD为2.070 0％.测得茯砖茶中芦丁含量为0.143 5％,RSD为3.490 0％.该方法简便、快速、准确,可用于茯砖茶中芦丁含量的测定.
语种：
中文
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Construction of Gene Library of 20 kb DNAs from Parasporal Crystal in Bacillus thuringiensis Strain 4.0718: Phylogenetic Analysis and Molecular Docking
作者：
Wu, Feng;Zhao, Xinmin;Sun, Yunjun;Li, Wenping;Xia, Liqiu* ;...
期刊：
Current Microbiology ,2012年64(2):106-111 ISSN：0343-8651
通讯作者：
Xia, Liqiu
作者机构：
[Wu, Feng; Li, Wenping; Yin, Jia; Zhao, Xinmin; Hu, Shengbiao; Yu, Ziquan; Sun, Yunjun; Xia, Liqiu; Ding, Xuezhi; Tang, Ying] Hunan Normal Univ, Hunan Prov Key Lab Microbial Mol Biol, State Key Lab Breeding Base Microbial Mol Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Zhao, Xinmin] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.
通讯机构：
[Xia, Liqiu] H;Hunan Normal Univ, Hunan Prov Key Lab Microbial Mol Biol, State Key Lab Breeding Base Microbial Mol Biol, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
关键词：
Bacillus thuringiensis;20 kb DNAs;Gene library;Homology analysis;Phylogenetic analysis;Molecular docking
摘要：
The 20 kb DNAs are associated with crystals in many subspecies of Bacillus thuringiensis. We isolated 20 kb DNAs from crystals of B. thuringiensis strain 4.0718, then constructed a gene library using DNA fragments of Sau3AI partial digestion and pbluescriptIISK(+) vector. We screened out 440 recombinants, yielding a genomic coverage of ten and including 99% sequence of DNA which achieved the required theoretical value to construct the gene library. Through NCBI Blast and homology analysis, the sequencing results proved that the DNA came from the chromosome of B. thuringiensis. Moreover, we have completed the multiple alignment of homologous ropB protein sequences and phylogenetic analysis using bioinformatic software. For further investigation of the interactions between 20 kb DNAs and protoxins, molecular docking has also been done.
语种：
英文
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Fast responsive fluorescence turn-on sensor for Cu2+ and its application in live cell imaging
作者：
Wang, Jiaoliang* ;Li, Hao;Long, Liping;Xiao, Guqing;Xie, Dan
期刊：
Journal of Luminescence ,2012年132(9):2456-2461 ISSN：0022-2313
通讯作者：
Wang, Jiaoliang
作者机构：
[Wang, Jiaoliang; Xie, Dan; Xiao, Guqing; Li, Hao; Long, Liping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构：
[Wang, Jiaoliang] H;Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
关键词：
Aqueous media;Bio-imaging;Color changes;Fast responsive;Fluorescent sensors;Image;Kinetic assay;Live-cell imaging;Naked-eye;Real time tracking;Hydrolysis;Sensors;Transition metal compounds;Transition metals;Fluorescence
摘要：
A new effective fluorescent sensor based on rhodamine was synthesized, which was induced by Cu 2 in aqueous media to produce turn-on fluorescence. The new sensor 1 exhibited good selectivity for Cu 2 over other heavy and transition metal (HTM) ions in H 2O/CH 3CN(7:3, v/v). Upon addition of Cu 2, a remarkable color change from colorless to pink was easily observed by the naked eye, and the dramatic fluorescence turn-on was corroborated. Furthermore, kinetic assay indicates that sensor 1 could be used for real-time tracking of Cu 2 in cells and organisms. In addition, the turn-on fluorescent change upon the addition of Cu 2 was also applied in bioimaging. © 2012 Elsevier B.V. All rights reserved.
语种：
英文
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