期刊论文

Long, L.-P.

中文

中国抗生素杂志

1001-8689

2011

36

8

606-609

10.13461/j.cnki.cja.004832

湖南省自然科学基金资助项目（07JJ6068）； 湖南省科技计划资助项目（2010HJ4123）；

本校为第一且通讯机构

目的 建立荧光光度法测定实际样品中铜离子的含量。方法 基于铜离子对阿莫西林的荧光有显著的熄灭作用，建立了荧光法快速测定铜离子含量的新方法。结果 铜离子与阿莫西林发生配合反应，配合物的组成比为1:2，将该方法用于水样中铜离子的测定，铜离子浓度的线性范围为1．00×10~(-6)~5．00×10~(-2)mol／L，检出限为1．87×10~(-7)mol／L。样品回收率为 94．0％-106．5％。结论该方法简便、快速、准确.

Objective We established a new method for quantitative determination of Cu^2＋. Method CuCu^2＋could quench the fluorescence intensity of amoxicillin. Based on this reaction, CuCu^2＋ was determined by fluorescence spectrophotometry. Results The cooperate reaction between amoxicillin and CuCu^2＋ took place, the composition of the complex between CuCu^2＋ and amoxicillin was 1：2. The reaction conditions were investigated in detail, The linear range of determination for Cu^2＋ was 1.00 ×10^-6-5.00 × 10^-2 mol/L, with detection limit of 1.87×...