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Fast responsive fluorescence turn-on sensor for Cu2+ and its application in live cell imaging

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成果类型:
期刊论文
作者:
Wang, Jiaoliang*;Li, Hao;Long, Liping;Xiao, Guqing;Xie, Dan
通讯作者:
Wang, Jiaoliang
作者机构:
[Wang, Jiaoliang; Xie, Dan; Xiao, Guqing; Li, Hao; Long, Liping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
通讯机构:
[Wang, Jiaoliang] H
Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Peoples R China.
语种:
英文
关键词:
Fluorescence;Copper(II);Hydrolysis;Image
期刊:
Journal of Luminescence
ISSN:
0022-2313
年:
2012
卷:
132
期:
9
页码:
2456-2461
基金类别:
Initially, sensor 1 displayed essentially no fluorescence in H2O/CH3CN, 7:3, v/v, indicating that the spirocyclic form was retained in the solution. However, treatment with Cu2+ immediately elicited a dramatic fluorescence turn-on response (<10 min) at room temperature (Fig. 1). A strong new emission band at 550 nm was formed, and up to about 70-fold enhancement in the fluorescence intensity was noted. The large fluorescence turn-on was corroborated by the observation that the emission color of
机构署名:
本校为第一且通讯机构
院系归属:
材料与化学工程学院
摘要:
A new effective fluorescent sensor based on rhodamine was synthesized, which was induced by Cu2 in aqueous media to produce turn-on fluorescence. The new sensor 1 exhibited good selectivity for Cu2 over other heavy and transition metal (HTM) ions in H2O/CH 3CN(7:3, v/v). Upon addition of Cu2, a remarkable color change from colorless to pink was easily observed by the naked eye, and the dramatic fluorescence turn-on was corroborated. Furthermore, kinetic assay indicates that sensor 1 could be used for real-time tracking of Cu2 in cells and organisms. In addition, the turn-on fluorescent change ...

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