黑茶中茶多酚的提取及其含量测定方法的研究进展
作者：
彭映林;谢丹;李媛;张劲
期刊：
广东化工 ,2019年46(19):110-111,138 ISSN：1007-1865
作者机构：
[谢丹; 彭映林; 张劲; 李媛] 湖南城市学院
关键词：
茶多酚;黑茶;提取;测定方法;含量
摘要：
茶多酚具有抗氧化、降血脂、降血糖、抗辐射等多种功能,在食品、医药、日化、保健等方面具有广泛的应用前景。本文综述了目前常用的几种黑茶中茶多酚的提取及含量测定技术,并对这些方法进行了阐述和比较,最后对茶多酚的提取及含量测定方法研究前景进行了展望。
语种：
中文
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基于石墨烯分子印迹电化学传感器测定芦丁
作者：
谢丹;龙立平;刘蓉;钟桐生;李媛;...
期刊：
分析科学学报 ,2017年33(4):545-548 ISSN：1006-6144
作者机构：
[谢丹; 龙立平; 刘蓉; 钟桐生; 李媛; 唐桥亭] 湖南城市学院材料与化学工程学院, 湖南, 益阳, 413000
关键词：
分子印迹传感器;循环伏安法;石墨烯;芦丁
摘要：
将石墨烯(GR)滴涂至裸Au电极表面,并以邻氨基酚为功能单体,芦丁为模板分子,制备了芦丁分子印迹膜电化学传感器,利用循环伏安法(CV)和差分脉冲伏安法(DPV)对制得的传感器进行了电化学性能研究,并且对制备条件和测定条件进行了优化。结果表明,与裸Au电极相比,该GR修饰的Au电极在[Fe(CN)_6]~(3-)/~(4-)溶液中峰电流明显增大,显著提高了芦丁分子印迹传感器的灵敏度。在最优实验条件下,基于 GR分子印迹电化学传感器在4. 40×10~(-6)~2. 80×10~(-4) mol/L范围内呈良好的线性关系,检测限为1.46×10~(-6) mol/L。用该传感器测定了黑茶中芦丁的含量,获得较好结果。
语种：
中文
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In Vivo Lighted Fluorescence via Fenton Reaction: Approach for Imaging of Hydrogen Peroxide in Living Systems
作者：
Liu, Changhui;Chen, Weiju;Qing, Zhihe* ;Zheng, Jing;Xiao, Yue;...
期刊：
Analytical Chemistry ,2016年88(7):3998-4003 ISSN：0003-2700
通讯作者：
Qing, Zhihe;Yang, Ronghua
作者机构：
[Liu, Changhui; Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Liu, Changhui; Wang, Lili; Xiao, Yue; Yang, Ronghua; Zheng, Jing; Li, Yinhui; Chen, Weiju] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Qing, ZH; Yang, RH] C;[Yang, Ronghua] H;Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
By virtue of its high sensitivity and rapidity, Fenton reaction has been demonstrated as a powerful tool for in vitro biochemical analysis; however, in vivo applications of Fenton reaction still remain to be exploited. Herein, we report, for the first time, the design, formation and testing of Fenton reaction for in vivo fluorescence imaging of hydrogen peroxide (H2O2). To realize in vivo fluorescence imaging of H2O2 via Fenton reaction, a functional nanosphere, Fc@MSN-FDNA/PTAD, is fabricated from mesoporous silica nanoparticle (MSN), a Fenton reagent of ferrocene (Fc), ROX-labeled DNA (FDNA), and a cationic perylene derivative (PTAD). The ferrocene molecules are locked in the pore entrances of MSN, and exterior of MSN is covalently immobilized with FDNA. As a key part, PTAD acts as not only the gatekeeper of MSN but also the efficient quencher of ROX. H2O2 can permeate into the nanosphere and react with ferrocene to product hydroxyl radical (·OH) via Fenton reaction, which cleaves FDNA to detach ROX from PTAD, thus in turn, lights the ROX fluorescence. Under physiological condition, H2O2 can be determined from 5.0 nM to 1.0 μM with a detection limit of 2.4 nM. Because of the rapid kinetics of Fenton reaction and high specificity for H2O2, the proposed method meets the requirement for real applications. The feasibility of Fc@MSN-FDNA/PTAD for in vivo applications is demonstrated for fluorescence imaging of exogenous and endogenous H2O2 in cells and mice. We expect that this work will not only contribute to the H2O2-releated studies but also open up a new way to exploit in vivo Fenton reaction for biochemical research. (Chemical Equation Presented). © 2016 American Chemical Society.
语种：
英文
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Silver nanoparticle gated, mesoporous silica coated gold nanorods (AuNR@MS@AgNPs): Low premature release and multifunctional cancer theranostic platform
作者：
Zhang, Zhehua;Liu, Changhui;Bai, Junhui;Wu, Cuichen;Xiao, Yue;...
期刊：
ACS Applied Materials & Interfaces ,2015年7(11):6211-6219 ISSN：1944-8244
通讯作者：
Zheng, Jing
作者机构：
[Liu, Changhui; Xiao, Yue; Bai, Junhui; Zheng, Jing; Yang, Ronghua; Li, Yinhui; Zhang, Zhehua; Tan, Weihong] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Wu, Cuichen; Tan, Weihong] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA.;[Wu, Cuichen; Tan, Weihong] Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, Gainesville, FL 32611 USA.
通讯机构：
[Zheng, Jing] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
mesoporous silica nanoparticles;gold nanorods;silver nanoparticle;GSH;photothermal therapy;photodynamic therapy
摘要：
Multifunctional nanoparticles integrated with an imaging module and therapeutic drugs are promising candidates for future cancer diagnosis and therapy. Mesoporous silica coated gold nanorods (AuNR@MS) have emerged as a novel multifunctional cancer theranostic platform combining the large specific surface area of mesoporous silica, which guarantees a high drug payload, and the photothermal modality of AuNRs. However, premature release and side effects of exogenous stimulus still hinder the further application of AuNR@MS. To address these issues, herein, we proposed a glutathione (GSH)-responsive multifunctional AuNR@MS nanocarrier with in situ formed silver nanoparticles (AgNPs) as the capping agent. The inner AuNR core functions as a hyperthermia agent, while the outer mesoporous silica shell exhibits the potential to allow a high drug payload, thus posing itself as an effective drug carrier. With the incorporation of targeting aptamers, the constructed nanocarriers show drug release in accordance with an intracellular GSH level with maximum drug release into tumors and minimum systemic release in the blood. Meanwhile, the photothermal effect of the AuNRs upon application to near-infrared (NIR) light led to a rapid rise in the local temperature, resulting in an enhanced cell cytotoxicity. Such a versatile theranostic system as AuNR@MS@AgNPs is expected to have a wide biomedical application and may be particularly useful for cancer therapy. © 2015 American Chemical Society.
语种：
英文
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Targeted Intracellular Controlled Drug Delivery and Tumor Therapy through in Situ Forming Ag Nanogates on Mesoporous Silica Nanocontainers
作者：
Liu, Changhui;Zheng, Jing;Deng, Li;Ma, Cheng;Li, Jishan;...
期刊：
ACS Applied Materials & Interfaces ,2015年7(22):11930-11938 ISSN：1944-8244
通讯作者：
Yang, Ronghua
作者机构：
[Deng, Li; Liu, Changhui; Yang, Sheng; Ma, Cheng; Zheng, Jing; Yang, Ronghua; Li, Yinhui; Li, Jishan] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Yang, Sheng; Yang, Ronghua] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Jinfeng; Wang, Jing] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Yang, Ronghua] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
drug delivery;mesoporous silica nanoparticle;DNA;silver sphere;glutathione
摘要：
Targeting nanocontainers to the pathological zone and controlling release of their cargoes, in particular delivery of anticancer drugs to specific tumor cells in a targeted and controlled manner, remain the key challenges in drug delivery. This paper reports the development of a traceable and tumor-targeted intracellular drug release nanocontainer. The nanocontainer is obtained by in situ growth of silver nanoparticles (AgNPs) on the surfaces of mesoporous silica nanospheres (MSNs) using a DNA-templated process. Additionally, drug release from the nanopores is achieved by selective glutathione (GSH)-triggered dismantle of the AgNPs, and the concurrent fluorescence change allows real-time monitoring of drug release efficacy and facile visualization of in vivo delivery events. After being functionalized with sgc8 aptamer on the outer shell of the AgNPs, the targeted nanocontainers are delivered into acute lymphoblastic leukemia cells by aptamer-mediated recognition and endocytosis. Moreover, the GSH-responsive process presents an improvement in the cell-specific drug release and chemotherapeutic inhibition of tumor growth. © 2015 American Chemical Society.
语种：
英文
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Study of the rice straw biodegradation in mixed culture of Trichoderma viride and Aspergillus niger by GC-MS and FTIR
作者：
Chen, Yaoning* ;Huang, Jingxia;Li, Yuanping;Zeng, Guangming;Zhang, Jiachao;...
期刊：
Environmental Science and Pollution Research ,2015年22(13):9807-9815 ISSN：0944-1344
通讯作者：
Chen, Yaoning
作者机构：
[Ma, Shuang; Chen, Yaoning; Huang, Aizhi; Huang, Jingxia; Zeng, Guangming; Zhang, Jie; Zhou, Wei; Xu, Wei; Li, Yuanping; Tan, Xuebin] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.;[Ma, Shuang; Chen, Yaoning; Huang, Aizhi; Huang, Jingxia; Zeng, Guangming; Zhang, Jie; Zhou, Wei; Xu, Wei; Li, Yuanping; Tan, Xuebin] Hunan Univ, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Hunan, Peoples R China.;[Li, Yuanping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.;[Zhang, Jiachao] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen, Yaoning] H;Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.
关键词：
Lignocellulose;Biodegradation;Mixed culture;Trichoderma viride;Aspergillus niger;GC-MS;FTIR
摘要：
This study was conducted to investigate the biodegradation ability of the mixed culture of Trichoderma viride and Aspergillus niger through the study of the organic matter extracted from rice straw and the lignocellulose structure by using gas chromatography–mass spectrometer (GC-MS) and Fourier transform infrared spectroscopy (FTIR). The results of the GC-MS showed that the mixed culture possessed shorter alkane (heptane) at the end of the incubation and more kinds of organic matter (except the alkanes, 29 kinds of organic matter were detected) than the pure cultures. It could be deduced that the organic matter could indicate the degradation degree of the lignocellulose to some extent. Moreover, pinene was detected in the mixed culture on days 5 and 10, which might represent the antagonistic relationship between T. viride and A. niger. The analysis of FTIR spectrums which indirectly verified the GC-MS results showed that the mixed culture possessed a better degradation of rice straw compared with the pure culture. Therefore, the methods used in this research could be considered as effective ones to investigate the lignocellulose degradation mechanism in mixed culture. © 2015, Springer-Verlag Berlin Heidelberg.
语种：
英文
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DNA-templated in situ growth of silver nanoparticles on mesoporous silica nanospheres for smart intracellular GSH-controlled release
作者：
Liu, Changhui;Qing, Zhihe;Zheng, Jing;Deng, Li;Ma, Cheng;...
期刊：
Chemical Communications ,2015年51(30):6544-6547 ISSN：1359-7345
通讯作者：
Yang, Ronghua
作者机构：
[Deng, Li; Liu, Changhui; Yang, Sheng; Ma, Cheng; Zheng, Jing; Yang, Ronghua; Li, Yinhui; Li, Jishan; Tan, Weihong] Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Changhui] Hunan City Univ, Dept Chem & Environm Engn, Yiyang 413000, Peoples R China.;[Yang, Sheng; Yang, Ronghua; Qing, Zhihe] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Jinfeng; Wang, Jing] Cent S Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha 410011, Hunan, Peoples R China.;[Tan, Weihong] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA.
通讯机构：
[Yang, Ronghua] H;Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
In this work, we propose a method for constructing silver nanoparticles (AgNPs) on mesoporous silica nanospheres (MSNs) by a DNA-templated process. By in situ formation, the gatekeeper can be easily modulated to meet different degrees of glutathione (GSH) stimuli for location-specific drug release.
语种：
英文
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Synthesis and Crystal Structure of Aromatic Hydrazone Compounds Containing Pyrazole
作者：
Ren, Tiegang;Liu, Shuyun;Li, Yongzhe;Li, Guihui* ;Wang, Jie
期刊：
Journal of Chemical Crystallography ,2014年44(7):346-351 ISSN：1074-1542
通讯作者：
Li, Guihui
作者机构：
[Ren, Tiegang; Li, Yongzhe; Wang, Jie] Henan Univ, Fine Chem & Engn Res Inst, Coll Chem & Chem Engn, Kaifeng 475004, Peoples R China.;[Liu, Shuyun] Hunan City Univ, Yiyang 413000, Peoples R China.;[Li, Guihui] Henan Univ, Key Lab, Minist Educ Special Funct Mat, Kaifeng 475004, Peoples R China.
通讯机构：
[Li, Guihui] H;Henan Univ, Key Lab, Minist Educ Special Funct Mat, Kaifeng 475004, Peoples R China.
关键词：
Aromatic hydrazone;Pyrazole;Synthesis;Crystal structure;Characterization
摘要：
Two novel aromatic hydrazone compounds containing pyrazole group were synthesized with 1-phenylpyrazole-4-carbaldehyde and benzene hydrazine (or p-tolylhydrazine hydrochloride) as the starting materials. As-synthesized aromatic hydrazone compounds were characterized by means of 1H NMR, FT-IR, Ms and single-crystal X-ray diffraction. Results shown that two aromatic hydrazone compounds crystallizes in the monoclinic system of space group P21/n for A and Pc for B respectively. Graphical Abstract: [Figure not available: see fulltext.] © 2014 Springer Science+Business Media New York.
语种：
英文
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Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting
作者：
Chen, Yaoning* ;Zhou, Wei;Li, Yuanping;Zhang, Jiachao;Zeng, Guangming;...
期刊：
Applied Microbiology and Biotechnology ,2014年98(9):4233-4243 ISSN：0175-7598
通讯作者：
Chen, Yaoning
作者机构：
[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.;[Chen, Yaoning; Huang, Aizhi; Zeng, Guangming; Huang, Jingxia; Zhou, Wei; Li, Yuanping] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China.;[Li, Yuanping] Hunan City Univ, Coll Chem & Environm Engn, Yiyang 413000, Hunan, Peoples R China.;[Zhang, Jiachao] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Chen, Yaoning] H;Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.
关键词：
Composting;PCR-DGGE;Denitrifying bacteria;RDA;Phylogenetic analysis
摘要：
The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high diversity of nir gene sequences within composting samples. RDA showed that the nirK and nirS gene structures were significantly related to pH and pile temperature (P < 0.05). Significant amounts of the variation (49.2 and 38.3 % for nirK and nirS genes, respectively) were explained by pH and pile temperature, suggesting that those two parameters were the most likely ones to influence, or be influenced by the denitrifiers harboring nirK and nirS genes. © 2014 Springer-Verlag.
语种：
英文
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