作者机构：
[Li, Zhihong; Zhou, Zhenhua] Hunan City Univ, Dept Phys Educ, Yiyang, Peoples R China.
会议名称：
2nd SREE Workshop on Medical Materials and Engineering, WMME 2012
会议时间：
SEP 15-16, 2012
会议地点：
Soc Resources Environm Engn, Hong Kong, PEOPLES R CHINA
会议主办单位：
Soc Resources Environm Engn
会议论文集名称：
Advances in Sciences and Engineering
关键词：
Neuropeptide Y (NPY);Calcitonin gene related peptide (CGRP);denervation;mobilization;Bone mineral density (BMD)
摘要：
This paper observed the changes of neuropeptide substance (calcitonin gene related peptide, CGRP; neuropeptide Y, NPY) level and its effects on bone mineral density, using the method of cutting off the nerves and fixing the hind legs. We found that: The content of NPY was highly related with BMD in denerved and immobilized groups. It can be observed as a key factor of Osteoporosis' formation. But the nerve integrity is a key for normal CGRP expression. Dynamic observation of changes of the CGRP content can forecast the formation and development of osteoporosis in a certain extent.
期刊：
Chinese Journal of Tissue Engineering Research,2009年13(33):6428-6432 ISSN：1673-8225
通讯作者：
Li, Z.-H.
作者机构：
[周振华; 李志宏] Department of Physical Education, Hunan City University, Yiyang 413000, Hunan Province, China;[谢菊英] Department of Rehabilitation, Xiangnan University Affiliated Hospital, Chenzhou 423000, Hunan Province, China
通讯机构：
[Li, Z.-H.] D;Department of Physical Education, , Yiyang 413000, Hunan Province, China
关键词：
SD
摘要：
BACKGROUND: Previous studies have showed that stimulating the sciatic nerve and central nerve injury may alter the content of neuropeptide Y (NPY), which influences the osteoclasts and bone metabolism. OBJECTIVE: To observe the effect of NPY on bone mineral density (BMD) after bilateral sciatic denervation and immobilization, and to perform the correlation analysis. DESIGN, TIME AND SETTING: Randomized controlled animal experiment was completed in the Affiliated Hospital of Xiangnan University from September to November in 2006. MATERIALS: Ninety-six male Sprague Dawley rats, aged 10 weeks, weighing 220-225 g, were used to prepare denervated models. METHODS: According to random number table, 96 rats were divided into 3 groups with 32 rats in each group. Circled digit oneThe denervated group: The bilateral sciatic nerve and femoral nerve were cut off in rats, dissected distally 5 mm, and then the incision was sutured. Circled digit twoImmobilized group: Rats were performed denervation surgery as above procedures, then immobilized with plaster cast. Circled digit threeIn the control group, only sham operation was performed, the nerves were exposed and then sutured. MAIN OUTCOME MEASURES: General conditions of the rats during experiment; eight rats were killed at 1, 10, 30, 60 days after the model preparation to determine the content change of NPY and bilateral BMD, as well as correlation between NPY and BMD. RESULTS: All 96 rats were included in the final analysis. Circled digit oneFollowing denervation, the content of NPY was decreasing at first and then increasing. With the prolong of denervation duration, the content of NPY soared up, exceeding normal levels. Circled digit twoIn the immobilized group, the content of NPY increased at first and bilateral then decreased. With the prolong of immobilization time, the content of NPY was shown to gradually increase and exceeded the normal levels. Circled digit threeWith the altering content of NPY, the BMD in bilateral tibial bone also changed and it decreased obviously at 30 days following denervation and immobilization. The NPY content was highly correlated with the BMD following denervation and immobilization (P < 0.01). CONCLUSION: The content of NPY is highly related with BMD in denervated and immobilized groups, so it can be observed as a key factor of osteoporotic formation.
期刊：
Chinese Journal of Tissue Engineering Research,2009年13(20):3933-3937 ISSN：1673-8225
通讯作者：
Li, Z.-H.
作者机构：
[李志宏; 周振华] Department of Physical Education, Hunan City University, Yiyang 413000, Hunan Province, China;[谢菊英] Deparment of Rehabilitation, Xiangnan University, Chenzhou 423000, Hunan Province, China
通讯机构：
[Li, Z.-H.] D;Department of Physical Education, , Yiyang 413000, Hunan Province, China
关键词：
降钙素基因相关肽;SD大鼠;失神经;固定;骨密度
摘要：
BACKGROUND: It is believed that calcitonin-gene-related peptide (CGRP) can stimulate osteoblast proliferation, increase bone mass, and promote nerve regeneration. However, the change rule, effect and mechanism of CGRP on osteoporosis formation after different immobilization remain unclear. OBJECTIVE: To observe the effect, as well as possible mechanism of neuropeptide on bone mineral density (BMD) after denervation and immobilization. DESIGN, TIME AND SETTING: The Randomized controlled animal experiment was completed in the Affiliated Hospital of Xiangnan University from September to November 2006. MATERIALS: Ninety-six male Sprague Dawley rats, weighing 220-225 g, were prepared denervated models. METHODS: According to random number table, 96 rats were divided into 4 batches, 3 groups in each batch, with 8 rats in each group. The denervated group: after anesthetized, femoral incision was made in the prone position on both sides of hind lateral, and bilateral sciatic nerve at the level of femur was cut off. Next, rats were turned to the supine position, longitudinal incision was made in the median of both sides, femoral nerve on both sides of rats at the level of inguinal ligament were cut of, dissected distally 5 mm, and then sutured incision. Immobilized group: rats were performed fixation and denervation surgery simultaneously, then immobilized 1, 10, 30, and 60 days with tude plaster after anesthetization. In the control group, only sham operation was performed. MAIN OUTCOME MEASURES: Ordinary circumstance of rat during experiment; the content change of CGRP and BMD as well as relevance between CGRP and BMD in 1, 10, 30, 60d after the model preparation. RESULTS: All rats were included in the final analysis Circled digit one After denervation, there was no initiative expanding or contracting activity with obvious decreased activity amount. Rats in the immobilized group had good spirits, with plucked hind limbs during movement. There was no obviously abnormal in the control group. Circled digit two Compared with the control group, the expression of CGRP was lower in the denervated group at days 1, 10, 30, and 60 after model preparation. (P < 0.05-0.01), which had statistical difference between the immobilized group and the control group in 30 days after immobilization (P < 0.05). The expression of CGRP was higher in the immobilized group than the denervated group at days 10 and 60 after model preparation (P < 0.05-0.01). Circled digit three Compared with the control group, BMD content in the immobilized group and the denervated group was decreased at days 30 and 60 after model preparation (P < 0.05-0.01), which began to decrease from 30 days, and obvious decreased at 60 days in the immobilized group and the denervated group, there was significant difference betweenintra-group comparison (P < 0.05-0.01). Circled digit four CGRP was highly related to BMD after denervation (P < 0.05). However, the correlative degree was not so high after immobilization. CONCLUSION: The nerve integrity is a key for normal CGRP expression. Dynamic observation of changes of the CGRP content can forecast the formation and development of osteoporosis in a certain extent.
