Immobilization of horseradish peroxidase to a nano-Au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide
作者：
Lei, CX;Hu, SQ;Shen, GL;Yu, RQ*
期刊：
Talanta,2003年59(5):981-988 ISSN：0039-9140
通讯作者：
Yu, RQ
作者机构：
[Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Coll, Dept Chem, Yiyang 413049, Peoples R China.;Zhuzhou Teachers Coll, Dept Chem, Zhuzhou 412007, Peoples R China.
通讯机构：
[Yu, RQ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
Nano-Au monolayer;Chitosan-entrapped carbon paste electrode;Hydrogen peroxide;Enzyme sensor
摘要：
A procedure for fabricating an enzyme electrode has been described based on the effective immobilization of horseradish peroxidase (HRP) to a nano-scaled particulate gold (nano-Au) monolayer modified chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups has been utilized to realize the use of nano-Au as an intermediator to retain high bioactivity of the enzyme. Hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) was determined in the presence of hydroquinone as a mediator to transfer electrons between the electrode and HRP. The HRP immobilized on nano-Au displayed excellent electrocatalytical activity to the reduction of H<inf>2</inf>O<inf>2</inf>. The effects of experimental variables such as the operating potential of the working electrode, mediator concentration and pH of measuring solution were investigated for optimum analytical performance by using an amperometric method. The enzyme electrode provided a linear response to hydrogen peroxide over a concentration range of 1.22 ×10<sup>-5</sup> -2.43 ×10<sup>-3</sup> mol 1<sup>-1</sup> with a sensitivity of 0.013 A 1 mol<sup>-1</sup> cm<sup>-2</sup> and a detection limit of 6.3 μmol 1<sup>-1</sup> based on signal per noise = 3. The apparent Michaelis-Menten constant (K<inf>m</inf><sup>app</sup>) for the sensor was found to be 0.36 mmol 1<sup>-1</sup>. The lifetime, fabrication reproducibility and measurement repeatability were evaluated with satisfactory results. The analysis results of real sample by this sensor were in satisfactory agreement with those of the potassium permanganate titration method. ©2003 Elsevier Science B.V. All rights reserved.
语种：
英文
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荧光熄灭的苦味酸光化学传感器
作者：
Long, LP*;Wang, KM;Yang, RH;Yang, XH
期刊：
分析化学,2003年31(4):414-419 ISSN：0253-3820
通讯作者：
Long, LP
作者机构：
[Long, LP] Hunan City Coll, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Peoples R China.
通讯机构：
[Long, LP] H;Hunan City Coll, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.
关键词：
荧光熄灭;苦味酸;光化学传感器;二苄基四甲基联苯二胺;辛可宁;2,4,6-硝基酚
摘要：
苦味酸对固定于聚氯乙烯(PVC)膜中的N,N′-二苄基-3,3′,5,5′-四甲基联苯二胺(TMBD)有可逆荧光猝灭作用,据此研制成测定苦味酸浓度的荧光化学传感器.该传感器敏感膜最佳组成为:50 mg PVC、100 mg 癸二酸二异辛脂(DOS)和6.0 mg TMBD.在0.12 mol/L H2SO4底液中测定苦味酸,其线性响应范围为2.10×10-7～4.00×10-4 mol/L, 检出限为7.8×10-8 mol/L, 其响应时间小于40 s.该传感器具有良好的重现性、可逆性和选择性,其它常见阴离子和阳离子不干扰测定.本方法用来直接测定合成水样中的苦味酸及间接测定药物辛可宁的含量,结果与文献方法一致.
语种：
中文
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Amperometric immunosensor for Schistosoma japonicum antigen using antibodies loaded on a nano-Au monolayer modified chitosan-entrapped carbon paste electrode
作者：
Lei, CX;Gong, FC;Shen, GL;Yu, RQ*
期刊：
Sensors and Actuators B-Chemical,2003年96(3):582-588 ISSN：0925-4005
通讯作者：
Yu, RQ
作者机构：
[Yu, RQ] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan City Coll, Dept Chem & Environm Engn, Yiyang 413049, Peoples R China.
通讯机构：
[Yu, RQ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
amperometric immunosensor;nano-Au monolayer;chitosan-entrapped carbon paste electrode;Schistosoma japonicum
摘要：
A new strategy to construct amperometric immunosensor for Schistosoma japonicum antigen (SjAg) assay based on nano-size particulate gold (nano-Au) monolayer as sensing platform has been described. The nano-Au monolayer was obtained through chitosan-entrapped carbon paste electrode (CCPE). The high affinity of chitosan entrapped in CCPE for nano-Au associated with its amino groups facilitated the formation of nano-Au monolayer on the surface of CCPE. A sequential competitive immunoassay format was performed on the nano-Au monolayer supported by CCPE using S. japonicum antibody (SjAb) and SjAg as a model system. The assay comprised first loading of SjAb on nano-Au monolayer, then blocking in BSA solution, followed by a competitive incubation in the buffer containing the SjAg (analyte) and SjAg labeled with horseradish peroxidase (HRP) and finally the amperometric detection with hydroquinone as an enzymatic substrate. The dynamic concentration range for SjAg assay is 0.11-22.4μgml<sup>-1</sup> with a detection limit of 0.06μgml <sup>-1</sup>. The feasibility of regenerating nano-Au monolayer for consecutive assays was demonstrated by a simple chemical treatment after each determination. The simple manipulations of construction of nano-Au monolayer, low-cost and improved sensitivity are main features of proposed immunosensing method. ©2003 Elsevier B.V. All rights reserved.
语种：
英文
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