National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21675042]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2017JJ2018]
机构署名:
本校为第一且通讯机构
摘要:
A novel fluorescent probe was synthesized and studied. Fluorescent probe 1 undergoes a unique Pi induced hydrolytic reaction in DMSO- HEPES at physiological pH that produces a colorimetric change associated with a 76 nm red-shift in the UV-vis absorption maximum and up to a 210-fold enhancement in the fluorescence intensity. It has successfully been used to stain and record microscopy images of HeLa cells. Moreover, the mechanistic proposal that these spectroscopic changes are associated with the reaction of Pi and probe 1 to form 2-(2-hydroxyp...