期刊论文

中文

中国实验方剂学杂志

1005-9903

2011

17

1

5-8+13

10.3969/j.issn.1005-9903.2011.01.002

湖南省高等学校科学研究项目（06C218）；

本校为第一机构

目的:建立疏花石斛多糖提取优选方案.方法:采用传统水浸提法、纤维素酶法、超声辅助法和微波辅助法4种不同处理方法对疏花石斛多糖进行提取;多糖含量的测定用苯酚一硫酸法.结果:纤维素酶法较优方案为1:30料液比,50℃,pH 4.5,200 U·mL-1作用2 h;微波辅助法较优方案为料液比1:30、微波功率档位为中火、作用时间8 min预处理后,80℃浸提1.5 h;超声辅助法较优方案为料液比1:30、超声功率300 W、超声处理6 rain预处理后,80℃浸提1.5 h;传统水浸提法较优方案为料液比1:30、温度80℃浸提1.5 h.结论:纤维素酶法提取疏花石斛多糖效果最佳,是多糖提取时的首选方案,传统水浸提法宜结合超声辅助法和微波辅助...

Objective：To establish an optimum method of extraction of polysaccharide from Dendrobium henryi. Method： Tradition water leach, cellulose enzyme, ultrasonic assistant and microwave assistant extracting procedures were used for polysaccharide extraction, and the phenol-sulfuric reaction was used for the determination of polysaccharide. Result：The optimum condition of extraction of Dendrobium polysaccharide was as follows： solid to liguid ratid was 1 ： 30 with enzyme of 200 U ·mL^- 1 extracted for 2 hours at 50 ℃ , pH4.5 in cellulose enzym...