期刊论文

中文

广东化工

1007-1865

2020

47

10

22-23

10.3969/j.issn.1007-1865.2020.10.011

07A038:湖南省教育厅科学研究项目 2016TP1022:黑茶金花湖南省重点实验室科研项目

本校为第一机构

以水提醇沉法制得伏砖茶多糖,以乙酸酐制备乙酰化伏砖茶多糖并测定取代度,采用体外化学模型研究伏砖茶多糖对α-淀粉酶、α-葡萄糖苷酶抑制活性。实验结果表明:伏砖茶多糖的提取率为4.62%,含量为68.72%,乙酰化茯砖茶多糖的取代度为0.214。4.0 mg/mL的茯砖茶多糖和乙酰化茶多糖对α-淀粉酶的抑制率分别为92.3%和96.8%;0.18 mg/mL的茯砖茶多糖和乙酰化茶多糖对α-葡萄糖苷酶的抑制率分别为90.3%和92.2%。两种多糖对两种酶均具有剂量依赖性抑制活性,乙酰化茶多糖抑制活性高于非乙酰化茶多糖,说明乙酰化修饰能增加茶多糖的降血糖活性。

Hot water extraction and alcohol precipitation were used to prepare Fuzhuan tea polysaccharide.Acetic anhydride was used to get the acetylated Fuzhuan tea polysaccharides and the substitution degree is detected.To determine the hypoglycemic activity,the inhibitory activity of polysaccharides against amylase and glucosidase was studied by using in vitro chemical model.The results showed that the extraction yield and content of Fuzhuan tea polysaccharides were 4.62%,68.72%respectively,and the substitution degree is 0.214.The inhibitory rate of 4.0 mg/mL fuzhan polysaccharides against alpha-amyla...