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A portable multi-channel turbidity system for rapid detection of pathogens by loop-mediated isothermal amplification

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成果类型:
期刊论文
作者:
Chen, Zhu;Yang, Tong;Yang, Haowen;Li, Taotao;Nie, Libo;...
通讯作者:
He, N;Li, Song;Li, Zhiyang;Wang, Lijun
作者机构:
[Li, Song; Yang, Haowen; Li, S; Deng, Yan; Chen, Zhu; Nie, Libo; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat Collaborat Innova, Zhuzhou 412007, Peoples R China.
[Chen, Zhu; Yang, Tong; Yang, Haowen; He, Nongyue] Southeast Univ, Natl Demonstrat Ctr Expt Biomed Engn Educ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
[Yang, Haowen] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland.
[Li, Taotao] Hunan City Univ, Sch Mat & Chem Engn, Hunan Prov Key Lab Dark Tea & Jin Hua, Yiyang 413000, Peoples R China.
[Mou, Xianbo] Ningbo Univ, Med Sch, Ningbo 315211, Zhejiang, Peoples R China.
通讯机构:
[He, N; Li, S] H
[He, N] S
[Li, Zhiyang] N
[Wang, Lijun] G
[Wang, Lijun] C
语种:
英文
关键词:
H7N9;LEGIONELLA;LOOP-MEDIATED ISOTHERMAL AMPLIFICATION;OPTICAL DETECTOR;TURBIDIMETER
期刊:
Journal of Biomedical Nanotechnology
ISSN:
1550-7033
年:
2018
卷:
14
期:
1
页码:
198-205
基金类别:
NSFCNational Natural Science Foundation of China (NSFC) [61527806, 61471168]; Hunan Key Research Project [2017SK2174]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016T90403]; Economical Forest Cultivation and Utilization of Collaborative Innovation Center in Hunan Province [(2013) 448]; Scientific Research Project of Hunan Education Department [17C0486]
机构署名:
本校为其他机构
摘要:
This study aimed at developing a portable multi-channel turbidity system (21 cm in length, 15.5 cm in width and 11.5 cm in depth) by real-time loop-mediated isothermal amplification (LAMP) method for rapid detection of pathogens. The developed system herein includes temperature control unit, photoelectric detection unit, turbidity calibration unit, power management unit, human machine unit, communication unit and ARM-based microcontroller. The coefficient of variation for eight channels is less than 0.25% in noise analysis. Legionella bacteria (LEG) and H7 subtype virus (H7) were successively ...

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