期刊论文

中文

生态环境学报

1674-5906

2014

23

12

1980-1985

10.16258/j.cnki.1674-5906.2014.12.005

201304310:林业公益性行业科研专项 2015JJ4012:湖南省自然科学基金 2010SK2004:湖南省科技计划 13C118:教育厅项目

本校为第一机构

从蒌蒿(Artemisia selengensis)体中分离分泌吲哚乙酸(IAA)的内生耐镉(Cd)细菌将有助于构建有效的植物-微生物联合修复体系。本研究以分泌IAA和Cd耐性为筛选指标,采用研磨法从蒌蒿的根、茎、叶中分离能产生IAA的内生耐Cd细菌,并对其铅(Pb)、铜(Cu)、锑(Sb)的耐受性及对蒌蒿生长特性的影响进行研究,根据形态特性、生理生化测定和16S rDNA序列分析对目标菌株进行分类鉴定。结果表明,从蒌蒿体内分离获得2株分泌IAA能力较强的内生耐Cd细菌J_2和Y_5,J_2和Y5菌对Cd的耐受质量浓度均为90 mg·L~(-1),IAA的分泌量分别为23.108、15.192 mg·L~(-1);J_2菌能明显增加蒌蒿的株高、最长根长、平均根长、鲜质量...

To isolate indole acetic acid (IAA)-producing cadmium-resistant endophytic bacteria fromArtemisia selengensis is to help build an effective combined remediation system of plant and microorganism. Using IAA-producing and cadmium-tolerance as the screening indexes, isolated IAA-producing cadmium-resistant endophytic bacteria from the root, stem and leafofArtemisia selengensis by the grinding method, and determinated the tolerance of lead, copper, antimony and the effect of the strains on growth ofAremisia selengensis. Strains were identified base...