期刊论文

Study on 4 weeks medium intensity aeraobic exercise induce the difference expression of the proteomics in rat atrial muscle

中文

中国应用生理学杂志

1000-6834

2018

34

5

450-456,463

10.12047/j.cjap.5683.2018.102

湖南省科技厅计划项目(2007FJ3075) 江苏省教育厅高校自然科学研究面上项目(14KJB310002) 江苏高校运动人体科学品牌专业建设工程资助项目(TAPP).

本校为其他机构

目的:探讨中等强度有氧运动对大鼠心房肌蛋白质组及其基因差异表达的影响,为运动心脏重塑和慢性心血管疾病康复研究提供研究依据.方法: 20只雄性SD大鼠按照体重随机配对分为对照组、实验组( n = 10),实验组大鼠每次按照速度24 m·min~(-1)、持续训练40 min(负荷强度相当于60%~ 70%VO2max ),每周训练6 d,持续训练4周中等强度有氧运动.应用双向凝胶电泳技术( 2-DE)分离心房肌蛋白质点,串联飞行时间质谱仪技术鉴定电泳结果中表达量上调≥5倍以上,下调至1 /5以下的13个备选目标蛋白质点.并对其中6个目标蛋白质用逆转录-聚合酶链式反应( RT-PCR)技术检测其mRNA.结果:通过软件分析,实验组与对照组比较...

OBJECTIVE: To investigate the effect of moderate-intensity aerobic exercise on the differential expression of rat atrial muscle Proteomics and genes, which provide research basis for the rehabilitation of chronic cardiovascular diseases and exercise -induced cardiac remodeling research. METHODS: Twenty male SD rats were randomly divided into control group and experimental group (n=10) according to body weight. Rats in the experimental group were trained (6 days per week),which lasted for 4 weeks of moderate-intensity aerobic exercise at a rate of 24 m.min(-1) for 40 min (load intensity equival...