期刊论文

中文

环境科学

0250-3301

2017

38

11

4874-4881

10.13227/j.hjkx.201704008

51378190:国家自然科学基金 51521006:国家自然科学基金 51578222:国家自然科学基金 51579094:国家自然科学基金 13JJB002:湖南省自然科学基金 2016SK2045:湖南省科技厅重点项目 17K019:湖南省教育厅创新平台开放基金

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采用PCR-DGGE技术,研究农业废物稻草堆肥化过程中参与纤维素降解的糖苷水解酶GH6家族基因的变化,并使用冗余分析(redundancy analysis, RDA)和蒙特卡罗置换检验(Monte Carlo permutation test)研究了生物信息矩阵与环境因子矩阵间的相关性.结果表明,8个理化参数对GH6家族基因变化影响的相对大小为:pH>堆体温度>TN(total nitrogen)>TOM(total organic matters)>C/N(TOC/TN)>含水率>环境温度>WSC(water soluble carbon),8个理化参数共解释了83.1%的GH6家族基因变化,对GH6家族基因变化有重要影响,其中pH(P=0.002)、堆体温度(P=0.004)和TN(P=0.004)是影响GH6家族基因变化的显著因子,三者分别解释了2...

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was used to study the changes of glycoside hydrolase family 6 (GH6 family) genes during the composting of agricultural waste. In addition, a redundancy analysis (RDA) and Monte Carlo permutation test were applied to determine the relationship between the changes to the GH6 family genes and physico-chemical parameters. According to the relative magnitudes of the influence caused by these different parameters on the changes to GH6 family genes, the eight-ranked physico-chemical parameters were pH>pile temperature>...