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The walnut JrVHAG1 gene is involved in cadmium stress response through ABA-signal pathway and MYB transcription regulation.

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成果类型:
期刊论文
作者:
Xu, Zhenggang;Ge, Yu;Zhang, Wan;Zhao, Yunlin;Yang, Guiyan
通讯作者:
Zhao, YL;Yang, GY
作者机构:
[Zhao, Yunlin] Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan Province, 410004, China. zyl8291290@163.com
[Xu, Zhenggang; Zhang, Wan] Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan Province, 410004, China
[Xu, Zhenggang] School of Material and Chemical Engineering, Hunan City University, 518 Yingbin Road, Yiyang, Hunan Province, 413000, China
[Ge, Yu] College of Forestry, Hubei University for Nationalities, 39 Xueyuan Road, Enshi, Hubei, 445000, China
[Yang, Guiyan] Laboratory of Walnut Research Center, College of Forestry, Northwest A & F University, Yangling, Shaanxi, 712100, China. yangguiyan@yahoo.com
通讯机构:
[Yang, Guiyan] Laboratory of Walnut Research Center, College of Forestry, Northwest A & F University, Yangling, Shaanxi, 712100, China.
[Zhao, Yunlin] Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan Province, 410004, China.
语种:
英文
关键词:
Heavy metal stress;JrVHAG1 gene;ABA-signal pathway;Promoter;MYB transcription factor
期刊:
BMC plant biology
ISSN:
1471-2229
年:
2018
卷:
18
期:
1
页码:
19
文献类别:
WOS:Article
所属学科:
ESI学科类别:植物学&动物科学;WOS学科类别:Plant Sciences
入藏号:
WOS:000423061200001;PMID:29357825
基金类别:
Forestry Science and Technology Project of Hunan Province [XKL201731]; Science and Technology Project of Hunan Province [2016TP2007, 2016TP1014]; Special Financial Grant from the China Postdoctoral Science Foundation [2017 T100782]; Postdoctoral Science Foundation Project of Shaanxi Province [2016BSHEDZZ117]
机构署名:
本校为其他机构
摘要:
BACKGROUND: Vacuolar H(+)-ATPase (V-ATPase) is a vital protein complex involved in abiotic stress response in plants. The G subunit of Juglans regia (JrVHAG1) was previously identified as a drought tolerance-related gene involved in the ABA (abscisic acid)-signal pathway. Heavy metal stress is becoming a major detriment for plant growth, development, and production. In order to understand the role of JrVHAG1, the potential function mechanism of JrVHAG1 exposed to CdCl2 stress was confirmed in this study. RESULTS: Transcription of JrVHAG1 was induced by ABA and increased to 58.89-fold (roots) and 7.38-fold (leaves) and by CdCl2 to 2.65- (roots) and 11.42-fold (leaves) relative to control, respectively. Moreover, when treated simultaneously with ABA and CdCl2 (ABA+CdCl2), JrVHAG1 was up-regulated to 110.13- as well as 165.42-fold relative to control in the roots and leaves, accordingly. Compared to the wild type (WT) Arabidopsis plants, the transgenic plants with overexpression of JrVHAG1 (G2, G6, and G9) exhibited increased seed germination rate, biomass accumulation, proline content, and activities of superoxide dismutase (SOD) and peroxidase (POD) under ABA, CdCl2, and ABA+CdCl2 treatments. In contrast, the reactive oxygen species (ROS) staining, malondialdehyde (MDA) content, hydrogen dioxide (H2O2) content, as well as electrolyte leakage (EL) rates of transgenic seedlings were all lower than those of WT exposed to ABA, CdCl2 and ABA+CdCl2 stresses. Furthermore, a 1200 bp promoter fragment of JrVHAG1 was isolated by analyzing the genome of J. regia, in which the cis-elements were identified. This JrVHAG1 promoter fragment showed expression activity that was enhanced significantly when subjected to the above treatments. Yeast one-hybrid assay and transient expression analysis demonstrated that JrMYB2 specifically bound to the MYBCORE motif and shared similar expression patterns with JrVHAG1 under ABA, CdCl2 and ABA+CdCl2 stress conditions. CONCLUSIONS: Our results suggested that the JrVHAG1 gene functions as a CdCl2 stress response regulator by participating in ABA-signal pathway and MYB transcription regulation network. JrVHAG1 gene is a useful candidate gene for heavy metal stress tolerance in plant molecular breeding.
参考文献:
Abdallah IB, 2015, FOOD CHEM, V173, P972, DOI 10.1016/j.foodchem.2014.10.095
Chen YH, 2006, CELL RES, V16, P830, DOI 10.1038/sj.cr.7310099
Clough SJ, 1998, PLANT J, V16, P735, DOI 10.1046/j.1365-313x.1998.00343.x
DalCorso G, 2010, PLANT SIGNAL BEHAV, V5, P663, DOI 10.4161/psb.5.6.11425
Dartan G, 2015, ENVIRON MONIT ASSESS, V187, DOI 10.1007/s10661-015-4337-5

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