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Radical Scavenging by Acetone: A New Perspective to Understand Laccase/ABTS Inactivation and to Recover Redox Mediator

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成果类型:
期刊论文
作者:
Liu, Hao*;Zhou, Pandeng;Wu, Xing;Sun, Jianliang;Chen, Shicheng
通讯作者:
Liu, Hao
作者机构:
[Liu, Hao; Sun, Jianliang; Wu, Xing] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China.
[Zhou, Pandeng] Hunan City Univ, Sch Chem & Environm Engn, Yiyang 413000, Peoples R China.
[Chen, Shicheng] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA.
通讯机构:
[Liu, Hao] S
S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China.
语种:
英文
关键词:
laccase;ABTS;inhibition;radical scavenging;recovery
期刊:
Molecules
ISSN:
1420-3049
年:
2015
卷:
20
期:
11
页码:
19907-19913
基金类别:
Foundation of State Key Laboratory of Pulp and Paper Engineering [2015QN03]
机构署名:
本校为其他机构
院系归属:
材料与化学工程学院
摘要:
The biosynthetic utilization of laccase/mediator system is problematic because the use of organic cosolvent causes significant inhibition of laccase activity. This work explored how the organic cosolvent impacts on the laccase catalytic capacity towards 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in aqueous solution. Effects of acetone on the kinetic constants of laccase were determined and the results showed Km and Vmax varied exponentially with increasing acetone content. Acetone as well as some other cosolvents could trans...

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