期刊论文

中文

生命科学研究

1007-7847

2011

15

4

283-289

10.16605/j.cnki.1007-7847.2011.04.001

国家自然科学基金资助项目（30971413） 湖南科技大学博士启动基金资助项目（E51090）

本校为其他机构

脂质过氧化中间产物丙二醛(Malondialdehyde,MDA)在生物体内表现了广泛的生物毒性,MDA也是机体过度训练和运动性疲劳的重要生理指标.采用光学显微镜和透射式电子显微镜观察不同浓度MDA作用后海马神经元形态和超微结构的变化,并采用荧光分光光度法测定原代培养的海马神经元中Ca~(2+)-ATPase活性的变化和胞质游离钙离子水平的变化,探讨MDA对海马神经元形态和结构上的破坏及神经元钙离子稳态的影响.在光镜下可观察到MDA作用下神经元突触变短,胞体肿胀,出现细胞死亡或凋亡的形态特征;在电镜下可观察到线粒体结构的明显破坏,内膜上的嵴颗粒减少或消失;同时MDA还通过抑制质膜Ca~(2+)-ATPase的活性和其...

As a useful physiological index for over trained or exercise-induced fatigue, there were kinds of biological toxicity of malondialdehyde （MDA） produced in the process of lipid peroxidation. In this investigation, in order to observe the effects of MDA on the damages of hippocampal neuronal shapes and ultra-structure, and examine the calcium homeostasis in primary culture neurons, the microscope and transmission electronic microscope were applied for observing the changes of shapes and the transforms of ultra-structures, and also, the fluorosp...