Cyanobacteria (primarily Microcystis aeruginosa; MA) blooms, caused by eutrophic waters, have a significant and negative effect on human health and on the ecosystem as a result of blue-green algal toxins. In this study, a novel phytoplankton absorption spectral model was established using normalized MA and Scenedesmus obliquus (SO) absorption spectra as reference vectors to characterize the absorption properties of cyanobacteria in an inland lake (Dongting Lake, China). This model can be used to estimate the relative contributions of cyanobacte...