期刊论文

A Turn-On Near-Infrared Fluorescent Probe for Determination of Fe~(3+)and Antioxidant Capacity of Dark Teas

Qi Feng-Pei;Liu Chang-Hui

中文

分析化学

0253-3820

2019

47

1

112-118

10.19756/j.issn.0253-3820.181121

National University Student Innovation and Entrepreneurship Training Project [[2017]7]; Research Project on Innovation and Learning for College Students in the Ministry of Education [[2017]116]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21675042]; Hunan Natural Science FoundationNatural Science Foundation of Hunan Province [2018JJ2017]; Research Foundation of Education Bureau of Hunan Province, China [15B043]; Open Fund of Hunan Provincial Key Lab of Dark Tea and Jin-Hua [HC6120]

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以4-甲基喹啉与二醛缩合剂反应得到新型喹啉基花菁类衍生物CyQ,用~1H NMR、HRMS-ESI确证其结构。CyQ与半胱氨酸(Cys)通过卤素-巯基亲核取代和重排反应得到CyQ-Cys,而铁离子(Fe~(3+))可诱导CyQCys荧光信号明显增强,且荧光信号的变化与Fe~(3+)浓度在40 ~ 300 μmol/L范围内呈现良好的线性关系,线性回归方程为y = 1.0619x+0.01355 (R~2= 0.9910),检出限为10.4 μmol/L(S/N= 3) 。同时,利用黑茶金花可还原Fe~(3+)的性质,采用Fe~(3+)介导的“关-开”策略,建立了黑茶抗氧化活性的荧光分析方法。

A novel quinoline-based cyanine derivative of CyQ was facilely synthesized by the condensation reaction of 4-methyl quinoline and dialdehyde, and its structure was characterized by H-1 NMR and HRMS-ESI. Then, the target compound CyQ-cysteine (Cys) was obtained by halogen-mercapto nucleophilic substation reaction and then rearrangement reaction between CyQ and Cys. The results showed that ferric ion (Fe3+) could efficiently cause the fluorescence enhancement of CyQ-Cys, and the fluorescence intensity was linear with the Fe(3+ )concentration in the range of 40-300 mmol/L with a regression equati...