The objective of the paper was to discuss the anti-fatigue action of ocean water with different hardness on exhausted swimming mice. Mice were chosen and randomly divided into RO group, D25 group, D100 group, D300 group and D600 group with 20 mice for each group. The mice of the control group, RO group were fed with the 15 ml/kg common fresh water reverse osmosis water by gavage; 15 ml/kg ocean water of four different kinds of hardness were given to the 4 experimental groups by gavage. The experiment lasted for 30 days. Mice loaded swimming tim...