期刊论文

Study on Removal of Cd by Aspergillus fumigatus in Liquid Culture

Chen, Yaoning(cyn@hnu.enu.cn)

中文

湖南大学学报(自然科学版)

1674-2974

2017

44

12

127-134

10.16339/j.cnki.hdxbzkb.2017.12.020

国家自然科学基金资助项目（51378190,21276069,51579094）~~；

本校为其他机构

研究了一株高耐Cd的烟曲霉在实验室摇瓶培养条件下对不同浓度Cd的去除效果,测定了其在去除过程中菌株产谷胱甘肽(GSH)的变化.结果表明,在重金属Cd浓度为50 mg/L时,烟曲霉去除Cd效率最佳,达到55.5%的去除率,其产生的GSH浓度达到了0.89 µmol/mg.研究比较了不同环境条件下烟曲霉对Cd的去除效率,发现在温度30 ℃,pH 5.0,加液量120 mL,转速100 r/min,接种量0.4 mL($1.0 \times {10^7}$ CFU/mL)的培养条件下,烟曲霉能达到最佳去除Cd的效率.通过这种培养条件的优化,烟曲霉较初始条件下对50 mg/LCd的去除率提高了29.8%,培养体系中GSH浓度增加了0.42 µmol/mg.谷胱甘肽过氧化物酶(GPX)和谷胱甘肽还原酶(...

The removal efficiency of a Cd-resistant Aspergillus fumigatus under different concentrations of Cd in laboratory flask culture was studied, and the changes of glutathione (GSH) production in the strain were determined. The results showed that the removal rate of Cd was 55.5% when the concentration of Cd was 50 mg/L, and the concentration of GSH was 0.89 μmol/mg. The comparison of Cd removal efficiency by Aspergillus fumigatus under different conditions showed that the optimal condition was 30℃, pH value of 5.0, add fluid volume of 120 mL, speed of 100 r/min, and inoculation amount of...