期刊论文

中文

中国实验动物学报

1005-4847

2013

21

1

32-37

10.3969/j.issn.1005-4847.2013.01.007

30971413:国家自然科学基金 E51090:湖南科技大学博士启动基金

本校为其他机构

目的研究丙二醛（MDA）对原代培养的海马神经元胞质中钙离子稳态的破坏作用及可能的信号机制。方法以Fur2/AM为荧光指示剂，采用荧光分光光度法定量测定原代培养海马神经元胞质游离钙浓度变化。结果随着MDA浓度的升高和作用时间的延长，导致胞质中游离钙水平显著升高，破坏其钙稳态。MDA所导致的海马神经元胞质游离钙水平升高包括两个过程：100 μmol/L的MDA可使胞质［Ca~(2+)］_i水平在0~10 min内的早期渐进升高过程，经历中间大约5 min的平台期后，接下来15~30 min的晚期显著升高。以细胞膜电压依赖的Ca~(2+)通道抑制剂nimodipine抑制外钙内流后，可显著抑制晚期胞质［Ca~(2+)］_i水平的升高，...

Objectivie To investigate the effect of malondialdehyde on the calcium homeostasis disruption in primary cultured rat hippocampal neurons, their possible mechanisms and signaling pathways of to the effects. Methods The concentration of cytosolic free calcium （ [ Ca2＋ ]i） was determined by fluorescence speetrophotometry with Fur2/AM as an indicator. Results The levels of [ Ca2＋]i were increased with the increasing MDA concentrations in the primary cultured hippocampal neurons and the duration of time, and the calcium homeostasis was disturbe...