期刊论文

Screening of Eurotium cristatum producing ferulate esterase and optimization of enzyme producing conditions

中文

湖南城市学院学报(自然科学版)

1672-7304

2023

32

03

67-71

10.3969/j.issn.1672-7304.2023.03.0011

2022JJ50285:湖南省自然科学基金项目 2016TP1022:黑茶金花湖南省重点实验室项目

本校为第一机构

通过制备、初筛和复筛PDA(potato dextrose agar)平板培养基和液体培养基,从8种不同来源的冠突散囊菌中筛选出1株高产阿魏酸酯酶的优势菌种,并通过控制反应时间、酶添加量和缓冲溶液pH值,对该优势菌种进行...展开更多 通过制备、初筛和复筛PDA(potato dextrose agar)平板培养基和液体培养基,从8种不同来源的冠突散囊菌中筛选出1株高产阿魏酸酯酶的优势菌种,并通过控制反应时间、酶添加量和缓冲溶液pH值,对该优势菌种进行条件优化实验,以确定其最佳反应条件。实验结果表明:3株冠突散囊菌(C20G11、C20G10和PDAJ1)在粗筛培养基中的菌落生长速度占据优势;通过复筛,发现PDAJ1菌落生长速度在3株冠突散...

By preparing PDA plate medium,PDA liquid medium,primary screening medium and double screening medium,one strain with high ferulic acid esterase production was screened out from 8 kinds of different sources of Eurotium cristatum.The optimum reaction conditions of the dom...MORE By preparing PDA plate medium,PDA liquid medium,primary screening medium and double screening medium,one strain with high ferulic acid esterase production was screened out from 8 kinds of different sources of Eurotium cristatum.The optimum reaction conditions of the domin...