期刊论文

Extraction of Flavonoids from Perilla Frutescen Meal

中文

湖南城市学院学报(自然科学版)

1672-7304

2014

23

1

40-43

10.3969/j.issn.1672-7304.2014.01.011

湖南省教育厅科研基金资助项目（12C0579）； 国家大学生研究性学习和创新性实验计划科研基金资助项目（SJ201207）；

本校为第一机构

用乙醇浸提法从紫苏粕中提取黄酮类化合物,通过单因素及正交试验,分别考察了浸提时间、浸提温度、乙醇体积分数及料液比对提取率的影响.结果表明：对照样品的线性回归方程为A=0.004 9＋0.01 c,相关系数R=0.999 8;浸提时间2h、温度70℃、乙醇体积分数50％、固液比1∶12 g·mL＾-1时黄酮类化合物的得率最高为51.34 mg·g＾-1,加标回收率在94.40％-107.18％之间.原紫苏叶中的黄酮类化合物的平均得率为70.09mg·g＾-1,说明紫苏叶经超临界CO2提取挥发油后黄酮类物质的损失较少,该工作可以促进紫苏粕资源的综合利用.

Flavonoids were extracted by ethanol from Perilla frutescen meal （Perilla frutescen leaves after extraction of supercritical CO2 fluid）. The optimum technical conditions were determined as follows through single factor test and orthogonal experiments： 2 h extraction,50% vol ethanol, temperature at 70℃ and the ratio of solid to solvent 1：12 （g·mL^-1）. Flavonoid was determined by spectrophotometry with rutin as standard sample. The regression equation of standard curve for rutin was A=0.004 9＋0.01 c（R=0.999 8）. Under the optimal extrac...