期刊论文

Zhao, Y.

中文

环境工程学报

1673-9108

2014

8

11

5010-5014

国家林业公益性行业科研基金项目（201304310）； 湖南省科技计划项目（2010SK2004）； 湖南省教育厅项目（13C118）；

本校为第一且通讯机构

根际细菌的筛选研究有助于构建有效的植物-微生物联合修复体系。采用稀释平板法从蒌蒿根际土壤中分离耐Cd产酸的根际细菌,并对菌株Pb~(2+)、Cu~(2+)和Sb~(3+)的耐受性,分泌吲哚乙酸(IAA)、铁载体、解磷特性及对土壤中Cd的活化效果进行了测定,基于形态特性、生理生化测定和16S rDNA 序列分析对目标菌株进行了分类鉴定。结果表明,从蒌蒿根际土壤中分离到能耐50 mg/L镉浓度且产酸能力较强的根际细菌1株T_3,菌株T_3对Pb~(2+)、Cu~(2+)和Sb~(3+)均具一定的耐受性,能分泌一定量的IAA和铁载体,但不具解磷特性,能明显增加土壤中有效态镉的含量,经鉴定可初步确定为芽孢杆菌属(Bacillus sp.)。为进一步研究...

Screening and studying on rhizospheric bacteria laid a theoretical basis for building effective combined remediation system of plant and microorganism. Cadmium-resistant producing acid rhizospheric bacteria was isolated from rhizospheric soil of Artemisia selengensis by dilution-plate method. The tolerance of Pb2+, Cu2+, Sb3+, the features of IAA-producing, siderophores, phosphate solubilization as well as the activation effect of cadmium in soil of strain were determinated. Strain was identified based on morphological, physiological-biochemica...